有氧运动对大鼠急性心肌梗死后心肌纤维化的影响  

Effect of aerobics exercise on myocardial fibrosis after acute myocardial infarction in rat

作　　者：周大亮[1] 郝丹[1] 魏林[1] 张天舒[1] 李为民[2] 张欣[2] 于丽芳[3] Zhou Daliang Hao Dan Wei Lin Zhang Tianshu Li Weimin Zhang Xin Yu Lifang(Department of Cardiology, the First Hospital of Harbin City, Harbin 150001, China Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Chin Clinical Pharmacy Depatment, the First Affiliated Hospital, Heilongfiang University of Chinese Medicine, Harbin 150020, Chin)

机构地区：[1]哈尔滨市第一医院心内科,哈尔滨150001 [2]哈尔滨医科大学附属第一医院,哈尔滨150001 [3]黑龙江中医药大学附属第一医院临床药学室,哈尔滨150020

出　　处：《中国医师杂志》2017年第6期852-854,共3页Journal of Chinese Physician

基　　金：哈尔滨市第一医院人才引进基金项目（2013SYYRCYJ06-3）

摘　　要：目的观察有氧运动对大鼠急性心肌梗死后心肌纤维化的影响。方法实验大鼠24只，随机分为3组：假手术组（n=8），梗死模型组（n=8）、有氧运动组（n=8），除假手术组外，其余各组大鼠制作前壁心肌梗死模型。10周后，观察各组大鼠结缔组织生长因子（CTGF）、I型胶原蛋白（COLlal）、Ⅲ型胶原纤维（COL3a1）基因表达，以及massoon心肌染色胶原容积分数（CVF）。结果与假手术组相比，心肌梗死组大鼠CTGF、COLlal、COL3al基因表达、CVF明显增加，差异具有统计学意义（P〈0．05，P〈0．01）。与心肌梗死组相比，有氧运动组CTGF、COLlal、COL3al基因表达、CVF明显降低，差异具有统计学意义（P〈0．05）。结论有氧运动可以通过调控CTGF因子的表达，抑制胶原蛋白沉积从而减轻心肌梗死后心肌纤维化程度。Objective To observe the effect of aerobics exercise on myocardial fibrosis after acute myocardial infarction （AMI） in rat. Methods Twenty-four AMI Sprague Dawley （SD） rats were randomly assigned to sham-operated group （Sham）, AMI group and aerobics exercise treatment group （ET）. Except Sham, other groups of rats were underwent anterior wall myocardial infarction. After ten weeks, the myocardial mRNA level of connective tissue growth factor （CTGF）, collagen I （COLlal）, collagen m （ COL3al ） detected by real time-quantitative polymerase chain reaction （PCR） analysis and myocardial collagen volume fraction （CVF） was determined on Masson stained sections. Results Campared to Sham-op-erated group, AMI group the level of CTGF, COLlal, COL3al, and CVF were increased （P 〈0. 05 ,P 〈0. 01）. Campared to AMI and ET groups, the levels of CTGF, COLlal, COL3al, and CVF were decreased （P 〈 0. 05,P 〈 0.01 ）. Conclusions Aerobics exercise can reduced the myocardial fibrosis after AMI in rat.

关 键 词：运动(Exercise) 心肌梗死/并发症 心内膜心肌纤维化症/并发症 

分 类 号：R542.22[医药卫生—心血管疾病]