苎麻脱胶果胶复合酶的优选及其效果分析  

Screening on compound pectinase for ramie degumming and its effect analysis

作　　者：成莉凤[1] 刘正初[1] 冯湘沅[1] 汪启明[2] 李琦[1,3] 郑科[1] 杨琦[1] 段盛文[1] CHENG Lifeng LIU Zhengchu FENG Xiangyuan WANG Qiming LI Qi ZHENG Ke YANG Qi DUAN Shengwen(Institute of Bast Fiber Crops, Chinese Academy of Agriculture Sciences, Changsha, Hunan 410205, China College of Life Science, IIunan Agricultural University, Changsha, Hunan 410128, China Hunan Lerkam Biology Co., Ltd., gueyang, Hunan 414100, China)

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205 [2]湖南农业大学生物科学技术学院,湖南长沙410128 [3]湖南利尔康生物股份有限公司,湖南岳阳414100

出　　处：《纺织学报》2017年第6期64-68,共5页Journal of Textile Research

基　　金：湖南省自然科学基金项目(2016jj3126);中国农业科学院创新工程(ASTIP-IBFC08);中国农业科学院基本科研业务费专项(Y2016PT36);国家现代农业产业技术体系建设专项(CARS-19-E24);湖南省战略性新兴产业科技攻关和重大科技成果转化项目(2014GK1041)

摘　　要：为研发高效苎麻脱胶酶制剂,对筛选并保藏的苎麻脱胶高效菌种进行液态发酵,测定胞外酶的果胶酶活力、甘露聚糖酶活力和蛋白质含量等特征参数。通过苎麻酶法脱胶,从纤维表观形态学、质量损失率、残胶率等方面综合分析苎麻脱胶效果。结果表明:第N组和第G组的果胶酶活力较优,分别为61.79、13.69 IU/m L,比酶活力分别为4.47、0.93 IU/mg;第N组和第G组的纤维素酶活力仅为0.01、0.02 IU/m L;第N组和第G组苎麻质量损失率分别为20.30%和18.69%,残胶率分别为2.52%和4.21%,均接近实际工业化应用水平;第N组的单纤维线密度为5.31 dtex,束纤维断裂强度为4.8 c N/dtex,属于优质脱胶苎麻纤维。To develop efficient enzyme preparation used in ramie degumming, the efficient strains used in ramie bio-degumming subjected to liquid state fermentation, which were screened and accessed. The characteristic parameters of extracellular enzymes including pectinase activity, mannanase activity and protein content, etc. were further determined, and the comprehensive indexes of the ramie degumming system, such as fiber morphology, mass loss rate, residual gum rate, etc. , were analyzed. The results show that pectinase activities of the group N and the group G were 61.79 IU/mL and 13.69 IU/mL, respectively, and their special enzyme activities were 4. 47 IU/mg and 0. 93 IU/mg, respectively. Cellulase activities of the group N and the group G were only 0. 01 IU/mL and 0. 02 IU/mL, respectively. Their mass loss rate and residual gum rate of the group N and the group G were 20.30% and 18.69% , and 2.52% and 4.21% , respectively, which are close to those of strains in practical industry application. The single fiber fineness of the group N was 5.31 dtex, and the fracture strength of the bundle fiber was 4.8 cN/dtex, which belonged to high-quality degummed ramie fiber.

关 键 词：苎麻 脱胶 果胶酶 半纤维素 纤维形态 

分 类 号：TS121[轻工技术与工程—纺织材料与纺织品设计] TS123.2[轻工技术与工程—纺织科学与工程]