中国5个地区献血人群HCV基因型分布及进化分析  

The genotype distribution and phylogenetic analysis of HCV among blood donors in five regions of China

作　　者：张玉[1,2] 高瞻[1,2] 杨亚闪 何苗[1,2] ZHANG yu GAO Zhan YANG Yashan HE Miao(Institute of Blood Transfusion, Peking Union Medical College, Chinese Academy of Medical Sciences, Chengdu, Sichuan, 610052, China Sichuan Blood Safety and Blood substitute International Science and Technology Cooperation Base. Corresponding author：HE Miao)

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]四川血液安全和血液代用品国际科技合作基地

出　　处：《中国输血杂志》2017年第5期443-448,共6页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金面上项目(81572089)

摘　　要：目的了解国内不同地区献血人群中HCV基因型、亚型分布及系统发育情况以及不同地区人群中HCV流行病毒株。方法收集来自重庆、新疆乌鲁木齐、河南洛阳、四川绵阳和广西柳州5地无偿献血人群的抗-HCV阳性血浆标本802(人)份,采用逆转录巢式PCR对其HCV core基因片段扩增并测序;应用MEGA 6.0软件构建HCV分子进化树并对其做基因分型,用Arlequin和DNAsp软件对不同地区不同HCV基因型做群体动力学研究。采用SPSSStatistics17.0软来分析HCV基因亚型和人口统计学特征的相关性。结果 5个地区献血者抗-HCV阳性血浆标本获得HCV core基因片段成功扩增的比例为52.24%(419/892),其中重庆为47.37%(116/247)、乌鲁木齐为54.17%(104/192)、绵阳为64.35%(74/115)、洛阳为38.95%(67/172)、柳州为76.32%(58/76);所有阳性PCR产物经双向测序后均获得核苷酸序列,共检测出8个HCV基因亚型:HCV-1a 0.72%(3/419)、1b 59.90%(251/419),2a 16.95%(71/419),3a 4.53%(19/419)、3b 3.82%(16/419),6a 12.41%(52/419)、6e 0.95%(4/419)、6n 0.72%(3/419),未检测到HCV-4和5型。5地献血人群中比例较高的2种HCV基因亚型:重庆为1b 46.55%(54/116)、6a18.97%(22/52),乌鲁木齐为1b 67.62%(71/104)、2a 21.90%(22/104)、洛阳为1b 64.18%(43/67)、2a 29.85%(20/67),绵阳为1b 80.00%(60/75)、2a 10.67%(8/75),柳州为1b 41.38%(24/58)、6a 36.21%(21/58)。HCV基因亚型和年龄、民族之间有明显的差异(P<0.05)。结论本组我国5个地区献血人群HCV基因亚型丰度较高,以1b为主,2a次之,其余呈散在分布或未检出;不同地区人群的优势基因型均以1b比例为最高,其次分别是2a或6a。HCV基因亚型和年龄、民族存在相关性。Objective To investigate HCV genotype and subtype distribution and phylogenetic analysis in different regions of China, and HCV strain among population from different regions. Methods A total of 802 plasma sample,positive to anti-HCV, from Chongqing, Xinjiang Urumqi City, Luoyang, Henan Province, Mianyang, Sichuan Province and Liuzhou, Guangxi blood donors are collected, and then HCV Core gene fragment is amplified using reverse transcription nested PCR and sequenced; To build HCV molecular evolutionary tree using MEGA 6.0, and determine its genotype and subtype ; To perform the group dynamics research of HCV genotypes in different regions using Arlequin and DNAsp; To analyze the rela- tionship between HCV genotype and demographics using SPSS 17.0. Results The percentage of being successfully amplifi- cated HCV Core gene fragment from Anti-HCV positive plasma sample of donors from five regions was 52. 24% （419/892） ： Chongqing 47. 37% （116/247） , Urumqi 54. 17% （104/192） , Mianyang 64. 35% （ 74/115 ） , Luoyang 38. 95% （67/172） and Liuzhou 76. 32% （58/76） ;All positive PCR products were Bidirectional sequenced, and then nucleotide sequences were acquired. There are eight HCV subtypes： HCV-la O. 72% （3/419）, lb 59. 90% （251/419）, 2a 16. 95% （71/419）, 3a 4. 53% （ 19/419）, 3b 3. 82% （ 16/419）, 6a 12.41% （52/419）, 6e 0. 95% （4/419） and 6n 0. 72% （3/419）, and HCV- 4 and 5 were undetected. The top two HCV subtypes among blood donors of five regions： lb 46.55%（54/116）and 6a 18. 97%（22/52）in Chongqing; lb 67.62%（71/104） and 2a 21.90%（22/104） in Urumqi; lb 64. 18%（43/67） and 2a 29. 85%（20/67） in Luoyang; lb 80.00%（60/75） and 2a 10.67%（8/75） in Mianyang; lb 41.38%（24/58） and 6a 36. 21% （21/58）in Liuzhou. There is significant difference between HCV subtype and age and ethnicity （P〈0. 05） Conclusion China has an abundance of HCV subtypes among blood donors with mainly lb and then 2a, and the others are diffusively distr

关 键 词：丙型肝炎病毒 HCV基因 基因亚型 系统发育 群体遗传 献血者 重庆 乌鲁木齐 洛阳 绵阳 柳州 

分 类 号：R446.1[医药卫生—诊断学] R512.63[医药卫生—临床医学]