荧光定量检测梅毒筛查阳性献血者中生殖器溃疡病原体的研究  

Real-time PCR detection of genital ulcer disease(GUD) pathogens in syphilis positive blood donors

作　　者：高瞻 张玉 杨亚闪 徐敏 何苗 GAO Zhan ZHANG Yu YANG Yashan XU Min HE Miao.(Institute of Blood Transfusion, Peking Union Medical College, Chinese Acad- emy of Medical Sciences, Chengdu 610052, China.)

机构地区：[1]中国医学科学院北京协和医院学院输血研究所,成都610052

出　　处：《中国输血杂志》2017年第5期470-472,共3页Chinese Journal of Blood Transfusion

摘　　要：目的研究梅毒筛查阳性献血者中生殖器溃疡病原体。方法收集梅毒筛查阳性献血者样本和人口地理学信息,提取核酸DNA,利用荧光定量技术检测病原体核酸。结果在1 679份样本中共发现37份梅毒DNA和33份HSV DNA阳性样品,其中HSV-1 20份,HSV-2 13份。梅毒螺旋体DNA阳性率为2.20%,HSV DNA阳性率1.97%其中HSV-1 1.19%和HSV-2 0.77%,并未发现H.ducreyi阳性样本。核酸阳性献血者多为男性,首次献血者,初中及初中以下文化程度。发现2份共感染样本。结论梅毒血液筛查不合格的献血者中存在一定感染生殖器溃疡病原体的风险应引起相关重视。Objective To detect GUD pathogens by real-time PCR in syphilis screening positive blood donors. Methods A total of 1 679 syphilis positive cases of whole blood and demographic information were eollected. DNA was extracted from plasma for real-time PCR to detect GUD pathogens. Results In real-time PCR test, there were a total of 37 T. patli- dum DNA positive samples and 33 HSV DNA positive with 20 HSV-1 and 13 HSV-2 respectively, but no H. ducreyi DNA was detected. The DNA positive rate of syphilis and HSV were 2. 20% and 1.97%, including HSV-1 at 1.19% and HSV-2 at 0. 77%. Most of the positive donors were males, first-time donors with middle school and below education. Only 2 DNA co-in- fected donors were found. Conclusion There is the risk of GUD pathogens infection in syphilis screening positive blood do- nors, and more attention should be paid to this current problem.

关 键 词：献血者 梅毒螺旋体 单纯疱疹病毒 杜克雷嗜血杆菌 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R446.1[医药卫生—临床医学]