悬浮红细胞在22-25℃的活性变化  

The active ingredients change of the suspended red blood cells at 22-25℃

作　　者：陈会欣[1] 王顺[1] CHEN Huixin WANG Shun(Department of Blood Transfusion, Wuhan First Hospital, Wuhan 430022, China)

机构地区：[1]武汉市第一医院输血科,湖北武汉430022

出　　处：《中国输血杂志》2017年第5期534-536,共3页Chinese Journal of Blood Transfusion

基　　金：武汉市卫生计生委医疗卫生科研资助项目(WX15B16);湖北省卫生计生科研基金资助项目(WJ2015CB006)

摘　　要：目的探讨悬浮红细胞离开输血科冰箱,在脱离冷链的状态下(即在室温,22-25℃),放置6个时间点其血液质量相关参数的变化。方法将待测悬浮红细胞血袋,在22-25℃室温下放置6个时间段(0 h、0.5 h、1 h、2 h、4 h、6 h),检测各时间点WBC、Hb、HCT、plt等指标的变化。结果随着脱离冷链时间的延长,悬浮红细胞的HCT在实验1组、实验2组、实验3组、实验4组和实验5组(53.33±4.19,52.25±4.17,53.19±4.28,52.11±4.23,53.07±4.20)%均明显低于对照组(53.39±4.15)%,P<0.05;悬浮红细胞中的WBC计数在各实验组(6.10±1.42,5.97±1.42,5.93±1.38,5.71±1.31,5.47±1.25)109/L均明显低于对照组(6.30±1.50)109/L,P<0.05;悬浮红细胞中的plt计数在实验3组,实验4组,实验5组(160.88±45.34,156.04±48.40,152.33±46.02)明显低于对照组(178.3±50.41),P<0.05;悬浮红细胞的Hb在实验3、4、5组(172.15±13.33,171.56±13.43,171.23±13.3)g/L均明显低于对照(172.49±13.19)g/L,P<0.05。结论悬浮红细胞脱离冷链后在22-25℃放置超过0.5h,WBC、HCT、plt均可出现明显下降,在22-25℃放置超过2h,血红蛋白含量出现明显下降。Objective To discuss the change in the quality of the suspended red blood ceils which left the refrigerator and out of the cold chain （22-25） and were observed at six points of time. Methods The suspended red blood cells were placed at 22 to 25℃ at room temperature, for six period of time （0 hour, 0. 5 hour, 1 hours, 2 hours, 4 hours, 6 hours）. At each point, the leukocyte red blood cells, hemoglobin and platelet count were measured. Results As the time out of the cold chain prolonged, the red blood cells deposited in each experimental group （53.39±4. 15, 52. 25±4. 17, 53. 19±4. 28, 52. 11±4. 23, 53.07±4. 20）% were significantly lower than the control group （53.39±4. 15）%, P〈0. 05. The leukocyte count of suspended red blood cells in each experimental group （6. 10±1.42, 5.97±1.42, 5.93±1.38, 5.71±1.31, 5.47± 1.25） 109/L were significantly lower than the control group （6. 30±1.50） 109/L, P〈0. 05. The platelet count of suspended red blood cells in each experimental group （ 171.02±45.31, 167.14±46. 39, 160. 88±45.34, 156.04±48.40, 178. 3±50. 41, 152. 33±46.02） were significantly lower than the control group （ 178. 3±50. 41, P〈0. 05）. The hemoglobin in sus- pended red blood cells in experimental group 3 （ 172. 15±13.33, 172.49±13.19） P〈0.05, experimental group 4 （ 172. 15 ±13.33, 171.56± 13.43） g/L, P〈0. 05, and experimental group 5 （ 172. 15± 13.33, 171.23 ± 13.36） g/L, P〈0. 05 were significantly lower than the control group. Conclusion Suspended red blood cells left out of cold environment and placed at 22 to 25 degrees Celsius for more than 0. 5 hours can result in a significant reduction in leukocyte and platelets count. If they are placed outside the cold chain at 22 to 25 degrees Celsius for more than 2 hours, the hemoglobin will appear significantly decreased.

关 键 词：悬浮红细胞 脱离冷链 活性成份 

分 类 号：R331.141[医药卫生—人体生理学] R457.12[医药卫生—基础医学]