乳胶凝集检测技术用于沙门菌快速筛选的研究  

Study on the application of latex agglutination test in the rapid detection of Salmonella in healthy physical examination

作　　者：杨宁[1] 张汉斌[2] 卢勉飞[1] 蔡芷荷[1] 吴清平[3] 

机构地区：[1]广东环凯微生物科技有限公司,广东广州510663 [2]广州市天河区疾病预防控制中心,广东广州510000 [3]广东省微生物研究所,广东广州510070

出　　处：《中国卫生检验杂志》2017年第11期1552-1553,1556,共3页Chinese Journal of Health Laboratory Technology

基　　金：广州市科技计划项目(201610010067)

摘　　要：目的评价乳胶凝集试剂盒对粪便标本中沙门菌可疑菌落的筛选性能。方法按照WS 271—2007《感染性腹泻诊断标准》的方法,从临床粪便标本中分离出104株可疑菌落,然后分别用沙门菌乳胶凝集试剂盒和三糖铁培养基进行初筛,最后用API 20E细菌鉴定条进行确认,分别从灵敏度及特异性方面对乳胶凝集试剂盒和三糖铁的性能进行评介。结果沙门菌乳胶凝集试剂盒筛查粪便标本分离的可疑菌落,灵敏度达到97.14%;特异度达到97.10%,远优于三糖铁测定的特异度(15.94%)。结论沙门菌乳胶凝集试验具有非常高的灵敏度及特异度,可以筛掉绝大多数的阴性菌落,而且对于目标菌落的识别率很高,是一种非常有效的快速筛选方法,值得推广应用。Objective To evaluate the screening performance of latex agglutination test kit for the detection of suspicious colo- nies of Salmonella from stool samples. Methods 104 suspected strains were isolated from clinical specimens according to WS 271--2007 Diagnostic Criteria for Infectious Diarrhea, and then Salmonella latex agglutination kit and Triple sugar iron medium were used for primary screening, finally, API 20E bacterial identification strip was used to confirm. The performances of latex agglutination kits and triple sugar iron medium were evaluated in terms of sensitivity and specificity. Results Salmonella latex agglutination kit was adopted to screen suspicious colonies isolated from fecal specimens, with sensitivity as 97.14%. The spe- cificity of the Salmonella latex agglutination kit reached 97.10% , which was better than that of the triple sugar iron（ 15.94% ）. Conclusion Salmonella latex agglutination test has high sensitivity and specificity, which can screen out most of the negative colonies. It has a high recognition rate for target colonies, it is a very effective rapid screening method, and it is worthy of popularization and application.

关 键 词：乳胶凝集检测技术 乳胶凝集检测试剂盒 沙门菌 健康体检 

分 类 号：R446.5[医药卫生—诊断学]