多重PCR在食源性微生物快速检验中的应用与评价  

Application and evaluation of multiplex PCR in rapid inspection of foodborne microorganisms

作　　者：吴科明[1] 黄飞[1] 李叶青[1] 石祖锟[1] 谢海[1] 蒙国伟 

机构地区：[1]防城港市疾病预防控制中心,广西防城港538021

出　　处：《中国卫生检验杂志》2017年第11期1557-1559,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的运用多重PCR技术对食品中的致病菌检测,确认该方法在食源性微生物检测的适用性和有效性,为食品中致病菌的检测提供快速、准确、有效的方法与决策依据。方法在防城港市防城区和港口区采集具有代表性样品对7种等常见致病菌检验、分析和评估,同时与传统分离培养方法进行对照研究。结果 290份样品分别用2种方法进行检测,传统培养法检出致病菌82株,致病菌检出率为28.3%,其中PCR法检出138份,致病菌检出率为47.6%,两者检出率差异有统计学意义(P<0.05)。结论多重PCR法同样适合不同类型食品的检测,有较强的高效性,灵敏度高,操作简便,适合检测成组病原微生物,对食源性致病菌做出初步的判断,再采用传统的培养法有针对性地加以确认,极大减轻了工作量和提高了工作质量,对及时判定病原菌、有效控制病原菌传播、预防食物中毒的发生具有重要意义。Objective The muhiplex PCR was used to detect the pathogens in food, and the applicability and validity of the method were confirmed. The method provided a rapid, accurate and effective method and decision basis for the detection of pathogenic bacteria in food. Methods Representative sample was collected for the detection, analysis and evaluation of 7 kinds of common pathogenic bacteria in Fangchenggang, and it was compared simultaneously with the traditional culture method. Results 290 samples were detected by two methods, the traditional culture method was used for the detection of 82 pathogens, the detection rate of pathogens was 28.3% , of which PCR detected 138 strains, with the detection rate of pathogen as 47.6% , with the differences of the detection rate statistically significant（ P 〈 0.05 ）. Conclusion Multiplex PCR method is also suitable for the detection of different types of food, which has strong high efficiency, high sensitivity, and easier operation, and is suitable for the detection of pathogenic microorganisms in groups; to make a preliminary judgment for the food borne pathogens, and then cultivation method is used for confirmation, which greatly reduces the workload and improves the quality of work, and is of great sig- nificance for the timely identification of pathogens, the effective control of the spread of pathogens, prevention of food poisoning.

关 键 词：多重PCR 分离培养 致病菌 对照研究 

分 类 号：R446.1[医药卫生—诊断学]