胶质瘤细胞H4中与多梳蛋白NSPc1相互作用的长链非编码RNA的鉴定  

Identification of lncRNAs interacting with polycomb protein NSPc1 in glioma H4 cells

作　　者：王玉梁[1,2,3] 梁之孔 李辉[5] 林彦琛 正午 龚燕华 

机构地区：[1]武警后勤学临床医学系院生物化学与分子生物教研室,天津300309 [2]武警8730部队医院,广东广州510800 [3]武警后勤学院附属医院神经内一科,天津300162 [4]河北医科大学基础研究院,河北石家庄050017 [5]武警后勤学院临床医学系人体解剖与组织胚胎学教研室,天津300309 [6]武警交通指挥部,北京100029

出　　处：《武警后勤学院学报（医学版）》2017年第2期113-117,130,共6页Journal of Logistics University of PAP（Medical Sciences）

摘　　要：【目的】鉴定胶质瘤H4细胞系中与多梳家族(polycomb group)蛋白NSPc1可能相互作用的长链非编码RNA(long noncoding RNA,lnc RNA)。【方法】在胶质瘤H4细胞系中,利用RNA结合蛋白免疫共沉淀RIP(RNA binding protein immunoprecipitation,IP)技术联合实时定量PCR技术在生物信息学预测与NSPc1蛋白结合评分较高的候选lnc RNAs中验证相互作用的lnc RNAs。【结果】鉴定出MALAT1、MEG3和SOX2OT可能与NSPc1蛋白相互作用。【结论】胶质瘤细胞中多梳蛋白NSPc1可能通过与lnc RNAs相互作用参与着肿瘤的发生发展、迁移、侵袭甚至肿瘤干细胞干性的维持。[Objective] To identify the long non-coding RNA （lncRNA） probably interacting with the Nervous System Polycomb 1 （NSPc 1） in glioma H4 cells. [ Methods ] RNA binding protein immunoprecipitation （RIP） associating real-time PC R technology were applied to screen lncRNAs among the candidates which had high scores in the NSPcl-lncRNA interaction evaluation by bioinformatics. [ Results ] The identification indicated that MALAT1, MEG3 and SOX2OT probably interacted with NSPcl. [Conclusion ] In glioma cells, NSPcl may be involved in the genesis, development, migration, invasion and even pluripotency of tumor stem ceils by interacting with lncRNAs.

关 键 词：胶质瘤 NSPC1 长链非编码RNA 

分 类 号：R34[医药卫生—基础医学]