CD8^+T细胞对白癜风患者黑素细胞的杀伤机制  

Mechanism of CD8^+T cells killing melanocytes in the patients with vitiligo

作　　者：马锦媛[1] 杨钰琪[1] 李舒丽[1] 郭伟楠[1] 宋璞[1] 安亚文[1] 高天文[1] 李春英[1] 

机构地区：[1]第四军医大学西京皮肤医院,陕西西安710032

出　　处：《中国麻风皮肤病杂志》2017年第6期341-343,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(编号:81502717;81602764;XJZT15M10)

摘　　要：目的:明确CD8^+T细胞对白癜风患者黑素细胞的杀伤机制。方法:分选白癜风患者及正常人外周血CD8^+T细胞,分别与原代黑素细胞及永生化正常黑素细胞系PIG1及永生化白癜风黑素细胞系PIG3V建立共孵育杀伤模型,采用流式细胞术检测靶细胞被杀伤情况,酶联免疫吸附法(ELISA)检测杀伤环境中IFN-γ、TNF-α、颗粒酶B及穿孔素的分泌水平。结果:白癜风患者外周血CD8^+T细胞杀伤原代黑素细胞及PIG3V能力,分泌IFN-γ、颗粒酶B及穿孔素水平均高于健康对照,而杀伤PIG1能力和TNF-α水平并无显著差异;白癜风患者CD8^+T细胞IFN-γ分泌水平与细胞杀伤率呈明显正相关。结论:白癜风患者CD8^+T细胞杀伤黑素细胞是白癜风发病的重要机制,且该杀伤作用可能依赖IFN-γ、颗粒酶B及穿孔素分泌水平的上调。Objective： To determine the killing mechanism of CD8＋T cells to melanocytes in the patients with vitiligo.Methods： CD8＋T cells were obtained from peripheral blood in 8 patients with vitiligo and 8 healthy controls.The primary melanocyte, immortalized normal melanocyte cell line PIG1 and immortalized vitiligo melanocyte cell line PIG3V were co-cultivated with selected CD8＋T cells respectively.The number of the death of melanocytes killed by CD8＋T cells was assessed by Flow cytometry.The expression levels of IFN-γ, TNF-α, granzyme B and perforin were measured by ELISA.Results： The number of died primary melanocytes and PIG3V killed by CD8＋T cells, the expression level of IFN-γ, granzyme B and perforin in the patients with vitiligo was higher than those in controls.There was no significant difference in the number of died PIG1 and the expression level of TNF-α between the patients and healthy controls.There was an obviously positive correlation between the level of IFN-γ secreted by CD8＋ T cells and the number of died melanocytes killed by CD8＋T cells.Conclusion： The killing of melanocytes by CD8＋T cells plays an important role in the development of vitiligo, which probably depends on the increased expression of IFN-γ, granzyme B and perforin.

关 键 词：白癜风 CD8+T细胞 干扰素γ 颗粒酶B 穿孔素 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]