南宁市102例HIV/HCV共感染者HCV基因型特征分析  

HCV genotypes among 102 HIV/HCV co-infection injection drug users in Nanning

作　　者：王亮[1] 刘海燕[1] 栾苑[1] 黄秋芳[1] 潘海西[1] 

机构地区：[1]南宁市疾病预防控制中心,广西南宁530023

出　　处：《中国热带医学》2017年第6期573-576,共4页China Tropical Medicine

基　　金：广西壮族自治区卫生厅自筹经费课题(No.Z2012646)

摘　　要：目的了解南宁市静脉注射吸毒人群(IDUs)中HIV/HCV共感染者HCV基因亚型流行情况。方法收集2012—2014年间南宁市静脉注射吸毒人员HIV/HCV共感染者血浆样本总共108份,设计特异性引物,通过巢式PCR对HCV NS5B区域长339 bp的片段进行扩增并测序,构建系统进化树对HCV进行基因分型。结果 108份血浆样本中巢式PCR成功扩增基因片段102份,系统进化分析显示检出4种HCV基因型含6种基因亚型,各基因亚型所占样本比例为6a 34.31%(35/102)、3b 33.33%(34/102)、1a 20.59%(21/102)、1b 6.87%(7/102)、3a 3.92%(4/102)和2a 0.98%(1/102)。在不同人口学特征时102份样本HCV基因型组成差异无统计学意义(P>0.05)。结论南宁市IDUs人群HIV/HCV共感染者中至少存在6种HCV基因亚型流行,6a、3b和1a为主要流行亚型。Objective To identify the prevalence of HCV subtypes among HIV/HCV co-infection injection drug users in Nanning City. Methods A total of 108 serum samples of HIV/HCV co-infection injection drug users were collected in Nanning City, from 2012 to 2014. The primers were designed and a 339 bp length fragment of HCV NS5B region was amplified by Nested-PCR, and sequencing was performed, and phylogenetic analysis was also progressed for HCV genotyping. Results Nested-PCR succeeded in amplifying 102 fragments from the 108 serum samples, and the phylogenetic analysis demonstrated four genotypes including six subtypes, and the distribution and constituent ratios of the subtypes were shown as fallowing： 6a （34.31%,35/102）, 3b （33.33%, 34/102）, la （20.59%,21/102）, lb （6.87%,7/102）, 3a （3.92%,4/102）, and 2a （0.98%, 1/102）. Chi-square test demonstrated that 102 samples of HCV gene subtype distribution showed no significant difference （P〉 0.05） in different demographic characteristics. Conclusion There are at least six HCV subtypes in the prevalence of HIV/ HCV co-infection injection drug users in Nanning City, and 6a, 3b and la are the most prevalence HCV subtypes.

关 键 词：静脉注射吸毒人群 丙型肝炎病毒 艾滋病病毒 共感染 基因分型 

分 类 号：R512.91[医药卫生—内科学]