Effect of diurnal temperature difference on lipid accumulation and development in Calanus sinicus(Copepoda:Calanoida)  

作　　者：周孔霖 孙松 

机构地区：[1]Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences [2]University of Chinese Academy of Sciences [3]Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology [4]Jiaozhou Bay Marine Ecosystem Research Station,Institute of Oceanology,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2017年第4期745-753,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the State Key Program of National Natural Science Foundation of China(No.41230963);the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11020305);the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(No.U1406403)

摘　　要：Calanus sinicus,the dominant copepod in the Yellow Sea,develops a large oil sac in late spring to prepare for over-summering in the Yellow Sea Cold Water Mass(YSCWM).The lipid accumulation mechanism for the initiation of over-summering is unknown.Here,we cultured C3 copepodites at four constant temperatures(10,13,16,and 19℃) and at three temperature regimes that mimicked the temperature variations experienced during diurnal vertical migration(10-13℃,10-16℃,and 10-19℃) for 18 days to explore the effects of temperature differences on copepod development and lipid accumulation.C.sinicus stored more lipid at low than at high temperatures.A diurnal temperature difference(10-16℃ and 10-19℃)promoted greater lipid accumulation(1.9-2.1 times) than a constant temperature of either 16℃ or 19℃,by reducing the energy cost at colder temperatures and lengthening copepodite development.Thereafter,the lipid reserve supported gonad development after final molting.Only one male developed in these experiments.This highly female-skewed sex ratio may have been the result of the monotonous microalgae diet fed to the copepodites.This study provides the first evidence that diurnal temperature differences may promote lipid accumulation in C.sinicus,and provides a foundation for future investigations into the mechanisms involved in over-summering in the YSCWM.Calanus sinicus, the dominant copepod in the Yellow Sea, develops a large oil sac in late spring to prepare for over-summering in the Yellow Sea Cold Water Mass （YSCWM）. The lipid accumulation mechanism for the initiation of over-summering is unknown. Here, we cultured C3 copepodites at four constant temperatures （10, 13, 16, and 19℃） and at three temperature regimes that mimicked the temperature variations experienced during diurnal vertical migration （10-13℃, 10-16℃, and 10-19℃） for 18 days to explore the effects of temperature differences on copepod development and lipid accumulation. C. sinicus stored more lipid at low than at high temperatures. A diurnal temperature difference （10-16℃ and 10-19℃） promoted greater lipid accumulation （1.9-2.1 times） than a constant temperature of either 16℃ or 19℃, by reducing the energy cost at colder temperatures and lengthening copepodite development. Thereafter, the lipid reserve supported gonad development after final molting. Only one male developed in these experiments. This highly female-skewed sex ratio may have been the result of the monotonous microalgae diet fed to the copepodites. This study provides the first evidence that diurnal temperature differences may promote lipid accumulation in C. sinicus, and provides a foundation for future investigations into the mechanisms involved in over-summering in the YSCWM.

关 键 词：Calanus sinicus diurnal temperature difference lipid accumulation OVER-SUMMERING 

分 类 号：S917.4[农业科学—水产科学]