CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development  

作　　者：Wang, Lingbo Li, Min-Yin Qu, Chao Miao, Wan-Ying Yin, Qi Liao, Jiaoyang Cao, Hua-Teng Huang, Min Wang, Kai Zuo, Erwei Peng, Guangdun Zhang, Shu-Xin Chen, Guodong Li, Qing Tang, Ke Yu, Qian Li, Zhoujie Wong, Catherine C. L. Xu, Guoliang[7] Jing, Naihe[6] Yu, Xiang[2,3] Li, Jinsong[1] 

机构地区：[1]Univ Chinese Acad Sci, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Inst Biochem & C, State Key Lab Cell Biol,Shanghai Key Lab Mol Andr, 320 Yueyang Rd, Shanghai 200031, Peoples R China [2]Univ Chinese Acad Sci, Chinese Acad Sci, Inst Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China [3]Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techn, 320 Yueyang Rd, Shanghai 200031, Peoples R China [4]Chinese Acad Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, 333 Haike Rd, Shanghai 201203, Peoples R China [5]Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China [6]Univ Chinese Acad Sci, Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China [7]Univ Chinese Acad Sci, Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, 320 Yueyang Rd, Shanghai 200031, Peoples R China [8]Nanchang Univ, Inst Life Sci, Nanchang 330031, Jiangxi, Peoples R China [9]Chinese Acad Sci, Inst Biochem & Cell Biol, Anim Core Facil, Shanghai 200031, Peoples R China

出　　处：《Cell Research》2017年第6期815-829,共15页细胞研究（英文版）

摘　　要：Studying the early function of essential genes is an Here, we established a method for rapidly inducing important and challenging problem in developmental biology. CRISPR-Cas9-mediated mutations in one blastomere of two- cell stage embryos, termed 2-cell embryo-CRISPR-Cas9 injection （2CC）, to study the in vivo function of essential （or unknown） genes in founder chimeric mice. By injecting both Cre mRNA and CRISPR-Cas9 targeting the gene of in- terest into fluorescent reporter mice, the 2CC method can trace both wild-type and mutant cells at different develop- mental stages, offering internal control for phenotypic analyses of mutant cells. Using this method, we identified nov- el functions of the essential gene Tet3 in regulating excitatory and inhibitory synaptic transmission in the developing mouse cerebral cortex. By generating chimeric mutant mice, the 2CC method allows for the rapid screening of gene function in multiple tissues and cell types in founder chimeric mice, significantly expanding the current armamentar- ium of genetic tools.

关 键 词：CRISPR-Cas9 two-cell embryo gene function Tet3 cerebral cortex neocortical development 

分 类 号：Q959.837[生物学—动物学] S814.8[农业科学—畜牧学]