Gas6在免疫性血小板减少症患者中表达增高及临床意义  

Increased Expression of Gas6 in Patients with Immune Thrombocytopenia and Its Clinical Significance

作　　者：李腾达 司宇 刘鹏 徐贵霞 谷明莉 叶辛[3] 张薇薇 黄元兰[4] 钱琤 邓安梅 

机构地区：[1]长海医院临床实验中心,上海200433 [2]长海医院检验科,上海200433 [3]复旦大学,上海200433 [4]解放军455医院,上海200052 [5]解放军100医院,江苏苏州215007

出　　处：《现代检验医学杂志》2017年第3期37-39,共3页Journal of Modern Laboratory Medicine

基　　金：973计划(2013CB531606);国家自然科学基金(81671556,81601406,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353);上海申康基金(SHDC22014014);上海教育科学基金(D14017);军队科研基金(BWS14J023,15ZD009,15XD007);美捷登基金(MJR20150019)

摘　　要：目的探究Gas6在免疫性血小板减少症(ITP)患者中的表达情况及其临床意义。方法以2013年10月~2015年12月于上海长海医院血液科确诊的35例ITP患者外周血作为实验组,以同期体检的35例健康个体外周血作为对照组,两组样本分离血浆后以酶联免疫吸附法(ELISA)检测Gas6,IFN-α,IL-4,IFN-γ和IL-17蛋白表达水平,组间比较采用两独立样本t检验,对Gas6与IFN-α等细胞因子的关系进行Pearson相关性分析。结果 Gas6在实验组与对照组的表达水平为27.28±7.56ng/ml vs 20.51±5.39ng/ml(t=4.314,P<0.0001);IFN-γ在实验组与对照组的表达水平为221.67±57.64 pg/ml vs 45.32±16.79 pg/ml(t=17.38,P<0.0001);IL-4在实验组与对照组的表达水平为113.86±26.48 pg/ml vs 49.87±14.98 pg/ml(t=12.44,P<0.0001);IL-17在实验组与对照组的表达水平为168.96±47.88 pg/ml vs109.56±28.97 pg/ml(t=6.28,P<0.0001);IFN-α在实验组与对照组的表达水平为34.83±8.12 pg/ml vs 29.89±5.76pg/ml(t=2.936,P=0.0045),差异均具有统计学意义(P<0.05)。Pearson相关性分析显示Gas6与IL-17,IL-4,IFN-γ呈正相关(r=0.564,0.486,0.449,P均<0.05),差异具有统计学意义,与IFN-α不具有相关性。结论 Gas6可能通过影响Th细胞亚群细胞因子的分泌参与了ITP致病过程,是临床上治疗和预测该病的潜在靶位点。Objective To investigate the expression level of Gas6 in patients with Immune Thrombocytopenia （ITP） and its clinical significance. Methods The experimental group was peripheral blood samples collected from 35 cases diagnosed with ITP in hematology department of Changhai Hospital in Shanghai from October 2013 to December 2015. Control group was peripheral blood from 35 healthy examined individuals at the same time. After separating plasm from the two group samples, the protein level of Gas6,IFN-α, IL-4, IFN-γ and IL-17 were measured by Enzyme-linked immunosorbent assay （ELISA）, comparison of expressional level of the two groups was measured by t test. Pearson correlation analysis was used to decide the relation between Gas6 and cytokines such as IFN-α. Results The expression level of Gas6 in experimental and control groups was 27.28±7.56 ng/ml vs 20.51±5.39 ng/ml （t=4. 314,P〈0. 000 1） ;IFN-γ was 22]. 67±57.64 pg/ml vs 45.32 ±16.79 pg/ml （t=17.38,P〈0. 000 1） ;IL-4 was 113.86±26.48 pg/ml vs 49.87±14.98 pg/ml （t= 12.44,P〈0. 000 1） IL-17 was 168.96±47.88 pg/ml vs 109.56±28.97 pg/ml （t=6.28,P〈0. 000 1） ;IFN-α was 34.83±8.12 pg/ml vs 29.89 ±5.76 pg/ml （t=2. 936,P=0. 004 5） ,all with statistical differences （P〈0. 05）. Pearson analysis showed that Gas6 was positively related with IL-I 7, IL-4, IFN-γ （r= 0. 564,0. 486,0.449, P〈0.05 ） and there was difference statistically, but Gas6 was not correlated with IFN-α. Conclusion Gas6 may participate in the disease formation of ITP through affection on cyto-kines secreted by Th cell subsets,and it was the potential therapeutic and predicted target for this disease clinically.

关 键 词：免疫性血小板减少症 生长捕获特异性蛋白6 白细胞介素-4 干扰素-Γ 白细胞介素-17 

分 类 号：R558.2[医药卫生—血液循环系统疾病] R392.11[医药卫生—内科学]