应用免疫组化方法检测天疱疮患者皮肤组织热休克蛋白70的临床研究  

Clinical Study on Heat Shock Protein 70 in Skin Tissue of Patients with Pemphigus by Immunohistochemistry

作　　者：黄芳[1] 靳耀峰[2] 卢杰[1] 李思鹏[1] 张婷[1] 冯义国[3] 陈静宏[4] 耿燕[1] 

机构地区：[1]西安交通大学第二附属医院检验科,西安710004 [2]西安交通大学第二附属医院病理科,西安710004 [3]西安交通大学第二附属医院皮肤科,西安710004 [4]西安交通大学环境与疾病相关教育部重点实验室暨卫生部微量元素与地方病重点实验室,西安710048

出　　处：《现代检验医学杂志》2017年第3期82-85,共4页Journal of Modern Laboratory Medicine

基　　金：陕西省卫生厅科研基金(2012D67)

摘　　要：目的通过对天疱疮患者皮肤组织热休克蛋白70(HSP70)蛋白表达水平的测定,探讨HSP70在皮肤组织表达与天疱疮发病的关系,为进一步阐明天疱疮的发病机制提供新的理论依据。方法采用免疫组化方法测定62例天疱疮患者皮肤组织HSP70,以正常皮肤组织作为对照进行HSP70测定。结果天疱疮患者水泡部位HSP70表达阳性细胞数>75%,相比正常皮肤HSP70的表达差异有统计学意义(Z=5.42,P<0.01),天疱疮患者炎性部位HSP70表达阳性细胞数>50%,相比正常皮肤HSP70的表达差异有统计学意义(Z=4.73,P<0.01)。结论天疱疮患者水泡部位以及水泡附近炎性细胞HSP70的异常增高表达,为HSP70参与天疱疮疾病的发生和发展提供了直接的证据。Objective This study was performed to investigate the levels of HSP70 in tissue in pemphigus as a possible new theoretical basis for further elucidate the pathogenesis of pemphigus. Methods The expression of HSP70 in 62 patients with pemphigus was determined by immunohistochemistry, and the normal skin was taken as control. Results The results showed that the positive cells of HSP70〉75% in the blisters of pemphigus vulgaris and the positive cells of HSP70〉50 in the inflammatory cells near the blisters,and the expression of HSP70 was significantly higher than that in normal skin, which was statistically significant（Z=5.42,4.73,P〈0.01）. Conclusion The abnormal expression of HSP70 in inflammatory cells and psoriasis of pemphigus patients showed that HSP70 is involved in the pemphigus.

关 键 词：天疱疮 热休克蛋白70 发病机制 

分 类 号：R758.66[医药卫生—皮肤病学与性病学] R446.8[医药卫生—临床医学]