罗氏cobase602与modular e170电化学发光分析仪检测血清HBsAg阳性低值结果的对比分析  

Study on the Low Positive Results of Detecting HBsAg in Serum Specimens with Roche Cobas e602 and Modular e170 Electrochemical Luminescence Analyzer

作　　者：李娅[1] 章迪 张赟[1] 皇海[3] 苏明权[3] 

机构地区：[1]西安市第四医院检验科,西安710004 [2]西安市中心血站,西安710061 [3]第四军医大学西京医院全军检验医学研究所,西安710082

出　　处：《现代检验医学杂志》2017年第3期123-125,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨乙肝表面抗原(HBsAg)阳性低值结果在不同检测系统间结果的相关性,为临床检验结果分析及报告的准确发布提供参考。方法选取经罗氏电化学发光检测仪Cobas e602检测的HBsAg结果在1~80 COI的标本243份,同时将标本在罗氏电化学发光检测仪Modular e170上进行检测,计算两组测定值的直线回归相关系数R^2值,计算各组别的阳性符合率。结果两个检测系统比对实验结果为R^2=0.933。根据测定值高低分为A,B,C,D和E组的阳性符合率分别为60.60%,92.72%,96.66%,96.66%和100%;男性组阳性符合率为87.66%,女性组阳性符合率为70.96%,男性患者阳性符合率大于女性患者(P<0.05)。结论两检测系统对于范围在1~80 COI的标本检测结果有相关性:其中测量值在10 COI以上标本的阳性符合率完全一致;测量值在1~10 COI的标本阳性符合率不一致,且随结果的减小而降低,在临床上可能导致有争议的结果,建议做进一步复查。Objective To investigate the correlation between the results of HBsAg positive results among different detection systems,and provide reference for the analysis of clinical test results and the publication of the report. Methods The HBsAg positive serum specimens with the quantitation result lower than 80 COI were detected by Roche Cobas e602 electrochemical luminescence analyzer. All the specimens were also detected by Roche Modular el70 electrochemical luminescence analyzer. The differences of detecting results were compared and performed the linear correlation analysis. Results The experimental results of two detection systems are Rz ： 0. 933. The positive coincidences of Group A, B, C, D and E were 60.60 %, 92.72 %, 96.66 %, 96.66 % and 100 %, respectively. The positive coincidence of the males was 87.66 %, while the positive coincidence of the females was 70.96 %. The positive coincidence of the males was significantly higher than the females （P〈0.05）. Conclusion The HBsAg detecting results of Roche Cobas e602 and Roche Modular el70 electrochemical luminescence analyzer had high correlation. The results higher than 10 COI had 100% positive coincidence rate,however the results between 1 and 10 COI were not. The result between 1 and 10 COI may lead to controversial results, suggestions for further checks.

关 键 词：乙肝表面抗原 罗氏Cobas e602 罗氏MODULAR e170 阳性低值 

分 类 号：R512.62[医药卫生—内科学] R446[医药卫生—临床医学]