乌司他丁干预对水下爆炸致兔急性肺损伤TNF-α表达的影响  

Effect of Ulinastatin on the Expression of TNF-αin the Rabbits with Acute Lung Injury Induced by Underwater Explosion

作　　者：孟凡凡[1] 齐晓林[1] 黄奕江[1] 叶长青[1] 王前进[1] 马宏昊[2] 邵先安[1] 

机构地区：[1]解放军第123医院检验科,安徽蚌埠233015 [2]中国科技大学近代力学系爆炸实验室,合肥230027

出　　处：《现代检验医学杂志》2017年第3期131-132,136,共3页Journal of Modern Laboratory Medicine

基　　金：南京军区医学重点课题资助项目(14ZD20)

摘　　要：目的探讨乌司他丁干预对水下爆炸致兔急性肺损伤时肿瘤坏死因子(tumor necrosis factor-alpha,TNF-α)的影响。方法健康新西兰兔随机分为爆炸损伤组与乌司他丁治疗组,以水下爆炸致兔急性肺损伤,用免疫酶联法检测爆炸后4,12和24 h血清中TNF-α浓度。以SPSS17.0统计软件进行分析,P<0.05为差异具有统计学意义。结果爆炸后4 h乌司他丁组TNF-α浓度为386.90±109.22ng/L,爆炸损伤组TNF-α浓度为538.20±201.43ng/L,二者相比无变化(t=2.088,P=0.051);爆炸后12h乌司他丁组TNF-α浓度为400.60±78.98 ng/L,爆炸损伤组TNF-α浓度为573.80±178.24ng/L,二者相比差异有统计学意义(t=2.809,P=0.012);24h乌司他丁组TNF-α浓度为356.10±130.99 ng/L,爆炸损伤组为552.30±169.64 ng/L,二者相比差异也有统计学意义(t=2.895,P=0.010)。结论乌司他丁干预下实验兔在爆炸12h后血清TNF-α降低。Objective The expression of TNF-α was detected in sera of rabbits treated by ulinastatin with acute lung injury induced by underwater explosion. Methods Rabbits were randomly divided into two groups such as the injured group and uli nastatin therapy group. Established underwater explosion device was used to cause acute lung injury in rabbits. TNF-α in sera of the rabbits were measured by ELISA at 4,12 and 24 hours after the explosion. The SPSS17.0 software was used to analyze the data and P〈0.05 was considered to be significant. Results There was no significant difference between the concentrations of TNF-α in the sera of rabbits in the injure group （538.20±201.43 ng/L） and that of in the ulinastatin group （386.90±109.22 ng/L,t=2. 088,P=0. 051） at 4 hours after burst. However,there was evidently decreased in the level of TNF a in the ulinastatin group （400.60!78.98 ng/L） compared with the injury group （573.80±178.24 ng/L,t=2. 809,P =0. 012） at 12 hours after burst. Moreover and TNF-α in the ulinastatin group （356.10±130.99 ng/L） was also decreased compared to the injure group （552.30±169.64 ng/L,t=2. 895,P=0. 010） at 24 hours after burst. Conclusion The TNF-α expression in sera of the rabbits in ulinastatin group were dramatically decreased than that of in injury group at 12 hours after burst,which may be benefit to rabbits with acute lung injury induced by underwater explosion.

关 键 词：水下爆炸 急性肺损伤 乌司他丁 肿瘤坏死因子-Α 

分 类 号：R332[医药卫生—人体生理学]