普城沙雷氏菌pigX基因的生物信息学分析  

Bioinformatics analysis of pigX gene in Serratia plymuthica

作　　者：吴岩[1,2] 邱旭华[2] 刘晓光[1,2] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学生命科学研究院,江苏镇江212013

出　　处：《江苏农业学报》2017年第3期510-516,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31240046);公益性行业(农业)科研专项(201503110-12)

摘　　要：通过生物信息学方法对普城沙雷氏菌(Serratia plymuthica)G3菌株中GGDEF/EAL结构域蛋白编码基因pigX启动子区域的调控信息以及编码蛋白质的理化特性和结构特点进行了解析。利用启动子分析软件Softberry和Neural Network Promoter Prediction对pigX基因编码区上游序列进行启动子预测,利用生物信息学方法对PigX蛋白的氨基酸组成和理化特性、磷酸化和糖基化位点、跨膜结构、信号肽二级结构和保守结构域等进行预测分析。结果显示:pigX启动子区有Fur和SoxS结合位点;PigX是微亲水性的脂结合蛋白,具有信号肽和跨膜结构域、糖基化和高水平磷酸化位点,α-螺旋和无规则卷曲是其主要二级结构。生物信息学分析预测结果表明G3菌株PigX具有较高水平磷酸化位点及由α-螺旋和无规则卷曲组成的二级结构,这与预测的PigX参与铁代谢和氧化应激反应及作为Csr D同源物与CSRB家族sRNAs分子结合的功能相吻合。To analyze the pigX gene encoding a GGDEF/EAL domain protein in Serratia plymuthica G3 strain, bioinformatics approaches was employed to unravel regulatory information of its promoter region and its nucleotide and amino acid sequences, as well as physicochemical characteristics, phosphrylation and N-glycosylation of PigX in strain G3 and the structure features such as transmembrane domain, signal peptide, secondary structure and conserve domain. Bioinformatics predictions identified that the binding sites of transcriptional regulators Fur and SoxS were located on the promoter of pigX, and PigX is a lipid-binding protein with a N-terminal signal peptide, trans-membrane domain and weak hydrophilicity. Fur-ther analysis revealed that PigX contains one N-glycosylation site and 23 phosphrylation sites withα-helix and random coil as its primary secondary structure, which is consistent with the predicted functions of PigX such as modulating iron metabo-lism and oxidative stress reaction, and binding small non-coding RNAs （ sRNAs） belonging to the CsrB family.

关 键 词：普城沙雷氏菌 GGDEF/EAL结构域 pigX基因 生物信息学 

分 类 号：S182[农业科学—农业基础科学]