KU-0063794抑制增生性瘢痕成纤维细胞增殖及迁移的研究  

Study of KU-0063794 on the inhibition of proliferation and migration of hypertrophic scar fibroblasts

作　　者：余州[1] 宋雅娟[1] 苏映军[1] 陈琳[1] 马显杰[1] 彭湃[1] 

机构地区：[1]第四军医大学西京医院整形外科,陕西西安710032

出　　处：《中国美容整形外科杂志》2017年第6期328-331,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金(30973752;81272118)

摘　　要：目的探索KU-0063794对增生性瘢痕成纤维细胞增殖及迁移能力的影响。方法分离培养人增生性瘢痕成纤维细胞,并进行形态学分析;采用无血清培养基饥饿培养增生性瘢痕成纤维细胞12~16 h,将其分为对照组和KU-0063794处理组。对照组给予DMSO处理;KU-0063794处理组给予不同浓度的KU-0063794(1.0、2.5、5.0、10.0、20.0μm)处理,24h后,采用MTS法和细胞划痕实验,分析KU-0063794对增生性瘢痕成纤维细胞增殖和迁移的影响。结果分离培养的增生性瘢痕成纤维细胞形态良好,KU-0063794呈浓度依赖性抑制增生性瘢痕成纤维细胞的增殖和迁移,其差异具有统计学意义(P<0.05)。结论成功分离培养人增生性瘢痕成纤维细胞,KU-0063794能够抑制人增生性瘢痕成纤维细胞增殖和迁移。Objective To explore the effect of KU-0063794 on the proliferation and migration of hypertrophic scar fibroblasts iso lated from human hypertrophic scar tissue.Methods Hypertrophic scar fibroblasts were isolated and cultured in vitro,and their morphological features were analyzed.The hypertrophic scar fibroblast cells were cultured in fetal bovine serum-free high-glucose Dulbecco Modified Eagle Medium for 12 to 16 hours,then divided into two groups：the control group （treated with DMSO） and KU-0063794 treated groups [treated with different doses of KU-0063794 （1.0,2.5,5.0,10.0,20.0 μm）] for 24 h.The Proliferation activity of hypertrophic scar fibroblasts was detected with an MTS kit.The migration ability of the hypertrophic scar fibroblasts was analyzed by wound healing assay.Results Isolated hypertrophic scar fibroblasts had good morphology.The proliferation and migration of hypertrophic scar fibroblasts could be inhibited by KU-0063794 in a concentration-dependent manner,and the difference was statistically significant （P＜0.05）.Conclusion Hypertrophic scar fibroblasts were successfully isolated.KU-0063794 had the ability to inhibit hypertrophic scar fibroblast proliferation and migration.

关 键 词：增生性瘢痕 成纤维细胞 KU-0063794 增殖 迁移 

分 类 号：R622[医药卫生—整形外科]