激活TRPV1在高同型半胱氨酸促内皮细胞凋亡中的作用  

Activation of TRPV1 in hyperhomocysteine-induced apoptosis of endothelial cells

作　　者：杨绍兵[1] 梁思敏[2] 曾祥飞[3] 黄政辉[3] 张秦宁 丛广志[3] 燕茹[2] 黄晖[2] 贾绍斌[4] 

机构地区：[1]宁夏医科大学总医院心脏中心,银川750004 [2]宁夏医科大学心脑血管病医院 [3]宁夏医科大学 [4]宁夏医科大学总医院心研所

出　　处：《临床心血管病杂志》2017年第6期587-591,共5页Journal of Clinical Cardiology

基　　金：宁夏医科大学优势学科群建设科研项目(No:XY201501);宁夏自然科学基金(No:NZ14113)

摘　　要：目的:研究激活TRPV1在高同型半胱氨酸促内皮细胞凋亡中的作用。方法:培养人脐静脉内皮细胞,用不同浓度同型半胱氨酸(0 mmol/L、0.05 mmol/L、0.1 mmol/L、0.2 mmol/L、0.5 mmol/L、1 mmol/L、2mmol/L、4mmol/L)及不同时间(6h、12h、24h、36h、48h)进行干预后CCK-8检测细胞增殖及活性情况,选择合适的干预浓度及干预时间;用免疫学方法检测TRPV1是否在人脐静脉内皮细胞上表达;Western blotting检测各组TRPV1蛋白的表达,TUNEL染色了解各组细胞凋亡情况。结果:(1)同型半胱氨酸对人脐静脉内皮细胞的增殖能力有明显的抑制作用,OD值从(1.03±0.12)渐下降至(0.42±0.05),且呈明显的剂量效应关系(P<0.01);同型半胱氨酸在0.5mmol/L开始、干预24h后对人脐静脉内皮细胞增殖有抑制作用(P<0.05);(2)细胞免疫荧光检测人脐静脉内皮细胞上有TRPV1表达,而对照组无表达;(3)不同浓度同型半胱氨酸干预细胞后TPRV1蛋白表达及细胞凋亡有差异(P<0.05);(4)辣椒素干预后TRPV1表达及细胞凋亡较单纯同型半胱氨酸干预组有统计学差异(P<0.05)。结论:高同型半胱氨酸可通过对TRPV1蛋白表达水平的下调,促进内皮细胞凋亡;激活TRPV1可抑制高同型半胱氨酸促内皮细胞凋亡。Objective：To investigate the role of TRPV1 in endothelial cell apoptosis induced by hyperhomocysteine.Method：After human umbilical vein endothelial cells were treated in different concentration and different time of homocysteine,CCK-8was used to measure the proliferation of HUVECs.TUNEL staining was used to detect the apoptosis.Western Blotting was used to measure the expression of TRPV1.Result：（1）Homocysteine significantly inhibited the proliferation of human umbilical vein endothelial cells,and the OD value decreased from（1.03±0.12）to（0.42±0.05）,P〈0.05;（2）The expression of TRPV1 and cell apoptosis were significantly different compared with control group,P〈0.05.Conclusion：Hyperhomocysteine can downregulate the expression of TRPV1 protein and promote the apoptosis of endothelial cells.Activating TRPV1 can inhibit the apoptosis of endothelial cells induced by hyperhomocysteine.

关 键 词：TRPV1 高同型半胱氨酸 人脐静脉内皮细胞 凋亡 

分 类 号：R541.4[医药卫生—心血管疾病]