CD36在慢性鼻-鼻窦炎伴鼻息肉组织中的表达与意义  

The expression of CD36 in nasal polyps with chronic sinusitis and its significance

作　　者：李军[1] 蒋春茂[1] 吴启宗[1] 黄江菊[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉头颈外科,400016

出　　处：《免疫学杂志》2017年第7期580-585,共6页Immunological Journal

摘　　要：目的炎症在慢性鼻-鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)的发生发展过程中起着重要作用,并且炎症在CRSwNP的病理机制仍然不完全清楚。此研究主要观察清道夫受体CD36在慢性鼻-鼻窦炎伴鼻息肉的表达。方法使用逆转录酶聚合酶链反应(RT-PCR),Western blot免疫印迹方法检测来自合计43例慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)或伴息肉患者的手术标本CD36的mRNA和蛋白表达。进一步通过免疫组织化学和免疫荧光技术观察CD36表达与分布规律。结果我们的RT-PCR和Western blot免疫印迹分析的结果表明,与正常鼻黏膜组织相比,鼻息肉标本中CD36 mRNA和蛋白表达显著增强(P<0.01)。免疫组织化学结果显示,CD36弱表达于慢性鼻窦炎鼻黏膜组织,而鼻息肉组织中表达较强。CD36与血管内皮标记物CD31的双染荧光结果显示,CD36阳性的细胞与CD31的阳性共标。结论研究数据表明CD36在慢性鼻-鼻窦炎伴鼻息肉发生与发展过程可能起着重要作用。Inflammation plays an important role in the development and progression of chronic rhinosinusitiswith nasal polyps（CRSw NP）, and the pathogenesis of inflammation in CRSw NP is not fully understood yet. Thisstudy was designed to observe the expression of scavenger receptor CD36 in chronic sinusitis with nasal polyps. Them RNA and protein expression of CD36 were detected by reverse transcription polymerase chain reaction（RT-PCR）and Western blotting in 43 patients with chronic sinusitis. At the same time, the expression and distribution of CD36 were observed by immunohistochemistry and immunofluorescence. RT-PCR and Western blot analysis showed thatCD36 m RNA and protein expression were significantly increased in CRSw NP compared with normal nasal mucosa（P〈0.05）; immunohistochemical results showed that CD36 was weakly expressed in squamous cell and epithelialcells of chronic sinusitis, while strongly expressed in CRSw NP. The results of double staining of CD36 and vascularendothelial markers CD31 showed co-expression of CD36 and CD31. These data suggest that CD36 may play animportant role in the development and progression of CRSw NP.

关 键 词：慢性鼻-鼻窦炎伴鼻息肉 炎症免疫 CD36 

分 类 号：R765[医药卫生—耳鼻咽喉科]