高效液相色谱法测定侗药马卡列丙中异绿原酸A和异绿原酸C的含量  

Determination of Isochlorogenic acid A and Isochlorogenic acid C in Duhaldea nervosa by HPLC

作　　者：关英 汪冶 周洋 王雨薇 郑斌婕 王李婷 蔡伟 

机构地区：[1]湖南医药学院药学院,湖南怀化418000 [2]湖南医药学院侗医药研究湖南省重点实验室,湖南怀化418000

出　　处：《时珍国医国药》2017年第5期1032-1034,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81603393);湖南省教育厅资助科研项目(No.16 B185);湖南医药学院博士启动基金(No.15001)

摘　　要：目的建立侗药马卡列丙中异绿原酸A和异绿原酸C的含量测定方法。方法高效液相色谱法采用Thermo Scientific Hypersil BDS C_(18)(250mm×4.6mm,5μm)色谱柱进行分析;流动相为0.1%甲酸水∶乙腈(梯度洗脱);流速为1.2 ml·min^(-1);检测波长为327 nm;柱温为40℃。结果异绿原酸A在2.54~50.8μg·ml^(-1)(r=1)范围内线性关系良好,平均加样回收率为104.3%,RSD为3.6%(n=6);异绿原酸C在1.28~25.7μg·ml^(-1)(r=0.9997)范围内线性关系良好,平均加样回收率为103.9%,RSD为1.7%(n=6)。结论该方法简便、准确,灵敏度高,重复性好,可用于侗药马卡列丙中异绿原酸A和异绿原酸C的含量测定,同时可为其质量控制提供参考依据。Objective To establish an HPLC method for the determination of isochlorogenic acid A and isochlorogenic acid C in Duhaldea nervosa. Methods Chromatographic separation was carried out with a Thermo Scientific Hypersil BDS C_（18）（ 250 mm ×4. 6mm,5μm） and a mixture of acetonitrile and 0. 01% formic acid with gradient elution as mobile phase. The flow rate was 1. 2m L·min-1,the detection wave length was 327 nm and the column temperature was 40 ℃. Results The linear range was 2. 54μg·m L-1~ 50. 8 μg·m L-1（ r = 1） for isochlorogenic acid A,and 1. 28 μg·m L-1~ 25. 7 μg·m L-1（ r = 0. 9997） for isochlorogenic acid C. The average recoveries of these compounds were 104. 3%（ RSD = 3. 6 %,n = 6） and 103. 9% %（ RSD =1. 7 %,n = 6）,respectively. Conclusion The established HPLC method is rapid,accurate,selective,and suitable for the determination of the content of isochlorogenic acid A and isochlorogenic acid C in Duhaldea nervosa.

关 键 词：马卡列丙 含量测定 异绿原酸A 异绿原酸C 

分 类 号：R284.1[医药卫生—中药学]