甘草次酸调节BLM的转录及PC3细胞的增殖凋亡和迁移侵袭  

Glycyrrhetinic acid regulating the transcription of BLM and proliferation,apoptosis,invasion and migration of PC3 cells

作　　者：陈福[1,2,3] 许厚强[1,2] 段志强[1,2] 赵佳福[1,2] 吴萍[1,2,3] 谌颖莲 

机构地区：[1]高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳550025 [2]贵州省动物遗传育种与繁殖重点实验室,贵州贵阳550025 [3]贵州大学生命科学学院,贵州贵阳550025

出　　处：《生物技术》2017年第3期282-288,共7页Biotechnology

基　　金：国家自然科学基金项目("RecQ解旋酶在前列腺癌细胞中的表达及作为抗癌药物靶标的研究";No.31361406)

摘　　要：[目的]探究甘草次酸(GA)影响前列腺癌(PC3)细胞BLM基因的转录以及细胞的增殖、迁移、侵袭和凋亡的情况。[方法]MTT法检测细胞增殖能力并筛选适宜的药物浓度,荧光定量PCR检测BLM基因的表达量,划痕法检测细胞迁移,Transwell小室检测细胞侵袭,流式细胞仪检测细胞凋亡。[结果]甘草次酸浓度在0.12 mol/L^0.36 mol/L范围时,随着浓度增大,细胞的增殖能力逐渐下降,呈现药物浓度梯度依赖性;当甘草次酸浓度为0.2 mol/L时,PC3细胞中BLM的mRNA的表达量仅有对照组的0.2倍;甘草次酸浓度在0.10 mol/L以上时,细胞的侵袭和迁移能力均显著减小;流式结果显示甘草次酸浓度在0.10 mol/L时,细胞凋亡率为45.25±0.5**,差异极显著(P<0.01)。[结论]甘草次酸浓度为0.15 mol/L以上时,能够下调PC3细胞中BLM基因的转录量(P<0.01),在0.12 mol/L^0.36 mol/L范围时,对PC3细胞的增殖、侵袭、迁移具有抑制作用,对PC3细胞凋亡具有促进作用。[ Objective ] In the current,the expression of BLM was detected and investigated the effect of glycyrrhetinic acid （GA） on transcription, cell proliferation, migration, invasion and apoptosis of prostate cancer （ PC3 ） cells. [ Methods ] Cell proliferation and drug screening of the suitable concentration had been evaluated by the MTT. The expression of BLM gene was detected by RT - qPCR, cell scratch was used to examine cell migration. Cell invasion was detected by Transwell and cell apop- tosis was assessed by flow cytometry. [ Results] The concentration of glycyrrhetic acid in 0.12 mol/L- 0.36 mol/L range ,with the increase of concentration, cell proliferation gradually decreased with concentration dependent. The concentration of glycyr- rhetic acid is 0.2 mol/L,0.2 times the expression level of PC3 cells in BLM mRNA was less than the control group. The con- centration of glycyrrhetic acid in the above 0.10mol/L, the migration and invasion of cells had been significantly reduced. Flow cytometry showed that the apoptotic rate was 45.25 ± 0.5 ＊ ＊ when the concentration of glycyrrhetinic acid was 0.10mol/L, the difference was significant （P 〈 0.01 ）. [ Conclusion] The proliferation, invasion and migration of PC3 cells were suppressed and promoted the apoptosis of PC3 cells when the concentration of glycyrrhetinic was acid in the range of 0.12 moL/L to 0.36 mol/L in PC3 cells ,when the concentration of glycyrrhetinie acid was 0.15 mol/L or more can down -regulated the BILM gene transcription.

关 键 词：BLM解旋酶 甘草次酸 前列腺癌细胞 

分 类 号：Q291[生物学—细胞生物学]