大丽轮枝菌拮抗芽孢菌株的分离、鉴定及两株菌抑菌特性研究  

Isolation and identification of antagonistic Bacillus spp.against Verticillium dahliae:the antibacterial properties of two strains

作　　者：赵君洁[1,2] 曾卫军[1] 李艳红[1] 葛风伟[1] 杜钰[1] 袁琳琳[1,2] 赵歉歉 王敏[1] 谢红桃[1] 白若翔 韩生成[2] 赵和平[2] 赵惠新[1] 

机构地区：[1]新疆师范大学生命科学学院新疆特殊环境物种保护与调控生物学实验室,新疆乌鲁木齐830054 [2]北京师范大学生命科学学院抗性基因资源与分子发育北京市重点实验室,北京100875

出　　处：《北京师范大学学报（自然科学版）》2017年第3期294-300,共7页Journal of Beijing Normal University(Natural Science)

基　　金：新疆维吾尔自治区高校科研计划科学研究重点资助项目(20140705081400714);新疆维吾尔自治区教育厅重点实验室"新疆特殊环境物种多样性应用与调控重点实验室"资助项目(XJTSWZ-2015-01);新疆师范大学博士启动基金资助项目(XJNUBS1416);抗性基因资源与分子发育北京市重点实验室开放基金资助项目(2015GD03)

摘　　要：从新疆和硕连作棉田根际土壤中分离到146株芽孢细菌.29株对大丽轮枝菌(Verticillium dahliae Kleb)具有拮抗作用的菌株中有9株拮抗性极强.经鉴定,9株拮抗性极强的菌株中有2株属短小芽孢杆菌(Bacillus pumilus),7株与枯草芽孢杆菌(B.subtilis)相近.抑菌特性实验表明:J-02和J-15的抑菌高峰分别出现在衰亡期和稳定期,对pH的耐受范围分别为5~9和7~9;J-02的热稳定性较好,J-15对温度较敏感,但100℃处理后仍有抑菌活性;J-02能够抑制大丽轮枝菌芽管伸长形成菌丝,J-15可导致大丽轮枝菌菌丝畸形,失去产孢能力.盆栽实验表明,J-02和J-15这2株拮抗菌对棉花黄萎病有明显防治作用.综合结果表明J-02和J-15均有作为棉花黄萎病生防菌的潜能.Cotton Verticillium wilt is the most serious fungal disease in Chinese cotton production, screening for its control for stable and high yields is of great significance in China. We have isolated antagonistic Bacillus strains against Verticilliurn dahliae from healthy continuously-cropping cotton rhizosphere soil in Xinjiang. A total of 146 strains were obtained and 29 strains showed varied degrees of antagonism to Verticilliurn dahliae. Of these strains J-02 and J-29 belonged to Bacillus pumilus and the remainder are similar to B. subtilis. We found that the strongest inhibition of J-02 on Verticillium dahliae was in decline phase, J-15 in stable phase and inhibition was stable in the pH range from 5~9 for J-02 and 7~9 for J-15 respectively. Strain J-02 was found to he more resistant to heat, J-15 was sensitive to temperature, but there was still an inhibition zone after 100 ℃ treatment J-02 inhibited the germ tube of Verticillium dahilae e- longates to form mycelium. J-15 caused mycelium of Verticillium dahliae to be deformed, and to fail to produce spores. In pot experiments, J-02 and J-15 showed a certain protective effect on cotton Verticillium wilt. In conclusion, strains J-02 and J-15 have potentials as biocontrol vectors against cotton Verticillium wilt.

关 键 词：棉花黄萎病 大丽轮枝菌 拮抗作用 芽孢杆菌 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]