Trichosporon montevideense WIN提取物合成纳米金及其对硝基芳烃的催化特性  

Biosynthesis of gold nanoparticles by the cell-free extracts of Trichosporon montevideense WIN for catalytic reduction of nitroaromatics

作　　者：裴晓芳[1] 沈文丽[1] 由胜男 厉舒祯 张照婧[1] 王经伟[1] 张旭旺[1] 曲媛媛[1] 

机构地区：[1]大连理工大学环境学院、工业生态与环境工程教育部重点实验室,大连116024

出　　处：《应用与环境生物学报》2017年第3期409-414,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(51508068);新世纪优秀人才支持计划(NCET-13-0077);中央高校基本科研业务费专项资金(DUT14YQ107)资助~~

摘　　要：利用生物及其提取物合成纳米金具有反应条件温和、环境友好等优点.以菌株Trichosporon montevideense WIN提取物合成纳米金(Au NPs),考察反应时间、p H和提取物浓度对纳米金合成的影响,并探讨纳米金催化还原硝基芳烃的特性.结果显示,纳米金合成过程可在7 d内稳定,最优p H为7.0,最适提取物浓度为300 mg/L.通过紫外-可见光谱扫描、透射电子显微镜和傅立叶转换红外线光谱分析可知,纳米金为球形和伪球形,尺寸分布范围为4-35 nm,平均粒径为(18.2±0.4)nm,推测提取物中的羟基、氨基和羧基参与了纳米金的还原和稳定过程.纳米金对2-硝基苯酚、3-硝基苯酚、4-硝基苯酚、2-硝基苯胺和3-硝基苯胺具有良好的催化活性,其催化反应速率常数分别为7.5×10^(-3)s^(-1)、6.4×10^(-3) s^(-1)、9.1×10^(-3) s^(-1)、11.8×10^(-3) s^(-1)和20.7×10^(-3) s^(-1).本研究表明T.montevideense WIN在生物合成纳米金方面具有潜在的应用价值,且合成的生物纳米金能够有效应用于硝基芳烃污染物的催化还原.Biological synthesis of gold nanoparticles （AuNPs） using organisms and their extracts has been attracting more and more attention owing to its advantages of mild conditions and environmentally friendly nature. In this study, cell-free extracts of Trichosporon montevideense WIN were used to biosynthesize AuNPs. Different parameters including reaction time, pH, and the concentration of extracts were optimized for the biosynthesis of AuNPs. UV-vis spectrophotometry, transmission electron microscopy （TEM）, and Fourier transform infrared spectroscopy （FTIR） were used to characterize the as-synthesized AuNPs. The reduction of nitroaromatics catalyzed by AuNPs was also investigated. The results showed that AuNPs synthesis by the cell-free extracts of strain WIN became stable within 7 days. The optimal conditions for the biosynthesis of AuNPs were pH 7.0 and 300 mg/L cell-free extracts. TEM images revealed that the AuNPs were spherical and pseudo-spherical with particle sizes ranging from 4 to 35 nm, and the average size of nanoparticles was found to be 18.2 ± 0.4 nm. FTIR spectra showed that hydroxyl, amide, and carboxyl groups might be involved in the formation of AuNPs. The biosynthesized AuNPs had excellent catalytic activities for reduction of 2-nitrophenol, 3-nitrophenol, 4-nitrophenol, 2-nitroaniline, and 3-nitroaniline, with catalytic rate constants of 7.5 × 10^-3 s-1, 6.4 × 10^-3 s-1, 9.1 × 10^-3 s-1, 11.8 × 10^-3 s-1, and 20.7 × 10^-3 s-1, respectively. The present study indicates that T. montevideense WIN may be a potential candidate for green synthesis of AuNPs that have high catalytic activities for reduction of nitroaromatics.

关 键 词：纳米金 生物合成 TRICHOSPORON montevideense 硝基芳烃 催化活性 

分 类 号：TB383.1[一般工业技术—材料科学与工程] X505[环境科学与工程—环境工程]