猪繁殖与呼吸综合征病毒乌鲁木齐分离株GP5基因遗传变异分析  

Genetic variation and phylogenetic analysis of glycoprotein 5 gene of pathogenic porcine reproductive and respiratory syndrome virus in Urumqi

作　　者：蔡扩军[1] 李爱巧[1] 何生 杨启元[1] 韩义忠 陈发喜[1] 梁望旺[4] 

机构地区：[1]乌鲁木齐市动物疾病控制与诊断中心,新疆乌鲁木齐830063 [2]乌鲁木齐市米东区畜牧兽医站,新疆乌鲁木齐831400 [3]乌鲁木齐市米东区三道坝畜牧兽医站,新疆乌鲁木齐831403 [4]重庆市动物疫病预防控制中心,重庆渝北401102

出　　处：《中国兽医杂志》2017年第5期20-22,共3页Chinese Journal of Veterinary Medicine

基　　金：新疆维吾尔自治区科技厅科技援疆项目(201491165);乌鲁木齐市科技局应用开发研究计划(Y141210013)

摘　　要：为对乌鲁木齐市猪繁殖与呼吸综合征病毒(PRRSV)的分子遗传变异情况进行研究与分析,应用RT-PCR方法对疑似感染PRRSV猪的组织脏器进行PRRSV GP5基因的扩增,将阳性样品PCR产物进行克隆和测序,并应用分子生物学软件对测序结果进行比对和遗传进化分析。结果显示,5株病毒分离株GP5基因全长均为603 bp,与JXA1株亲缘关系较近,同属于美洲株的同一基因亚群。本试验结果为掌握乌鲁木齐市PRRSV的分子遗传变异情况提供了依据。To study and analyze the molecular genetic variations of pathogenic porcine reproductive and respiratory syndrome virus（PRRSV） in Urumqi, the GP5 genes of suspected PRRSV positive samples were amplified by RT -PCR. PCR products of positive samples were cloned and sequenced. The results of alignment analysis showed that all the full length of GP5 from 5 isolates was 603bp, and they were closely related to the JXAlstrain and belonged to the same subgroup. This study would provide the basis for mastering the molecular genetic variation of PRRSV in Urumqi.

关 键 词：猪繁殖与呼吸综合征病毒 GP5基因 遗传变异 

分 类 号：S852.65[农业科学—基础兽医学]