SIRT1对急性肾损伤大鼠的抗纤维化作用  

An anti-fibrosis effect of silent information regulator 1 in rats with acute renal injury

作　　者：熊玉龙 姚宏伟[1] 许贤林[1] 

机构地区：[1]常州市第一人民医院泌尿外科,江苏省213000

出　　处：《江苏医药》2017年第11期757-760,I0001,共5页Jiangsu Medical Journal

摘　　要：目的探讨沉默信息调节因子1(SIRT1)对急性肾损伤的保护作用及其机制。方法用H_2O_2 400mmol/L(H1组)、800mmol/L(H2组)和1200mmol/L(H3组)以及H_2O_2 400mmol/L+白藜芦醇(Res)5mmol/L(H1+R组)、H_2O_2 800mmol/L+Res 5mmol/L(H2+R组)和H_2O_2 1200mmol/L+Res 5mmol/L(H3+R组)处理大鼠肾小管上皮细胞系NRK-52E细胞24h;设置正常培养的细胞作为对照组。用CCK-8法检测细胞活力,根据CCK-8实验结果选择H_2O_2 800mmol/L作用于NRK-52E细胞,同时用Res 5mmol/L(R组)处理细胞,24h后倒置显微镜下观察细胞形态,流式细胞仪检测细胞周期,Western blot检测SIRT1、转化生长因子(TGF)β1和结缔组织生长因子(CTGF)蛋白的表达。结果与对照组比较,H1、H2、H3组NRK-52E细胞活力降低(P<0.01),而加入Res 5mmol/L后,NRK-52E细胞活力增加(P<0.01)。H2+R组的细胞形态、数目优于H2组。H2组细胞G2/M期比例高于对照组和H2+R组(P<0.01)。与对照组比较,H2组SIRT1蛋白表达降低(P<0.01),R组SIRT1蛋白表达升高(P<0.01);H2组TGF-β1和CTGF蛋白表达较对照组和H2+R组升高(P<0.01)。结论 SIRT1对急性肾损伤具有抗纤维化的作用,其可能机制是通过降低异常增加的G2/M期细胞来发挥作用。Objective To investigate the protective effect and underlying mechanism of silent information regulator 1（SIRT1）on the rats with acute kidney injury（AKI）.Methods NRK-52 Ecells were treated with H2O2 400mmol/L（group H1）,800mmol/L（group H2）,1200mmol/L（group H3）and H2O2 400mmol/L＋resveratrol（Res）5mmol/L（group H1＋R）,H2O2 800mmol/L＋Res 5mmol/L（group H2＋R）,H2O2 1200mmol/L＋Res 5mmol/L（group H3＋R）for 24 hours.Normal cultured cells were used as the controls（group C）.The cell viability was detected by CCK-8assay.NRK-52 Ecells were treated with H2O2 800mmol/L according to the result of CCK-8assay,which were treated with Res 5mmol/L（group R）additionally.The cell morphology was observed under inverted microscope.The cell cycle was detected by flow cytometry.The protein expressions of SIRT1,transforming growth factor（TGF）-β1and connective tissue growth factor（CTGF）were detected by Western blot.Results Compared to group C,the cell viability was lower in groups of H1,H2 and H3（P〈0.01）,which was increased after treated with Res 5 mmol/L（P〈0.01）.The cell morphology and number were better in group H2＋R than those in group H2.The ratio of G2/M was higher in group H2 than that in groups of C and H2＋R（P〈0.01）.Compared to group C,the protein expression of SIRT1 was lower in group H2（P〈0.01）,which was higher in group R（P〈0.01）.The protein expressions of TGF-β1and CTGF were higher in group H2 than those in groups of C and H2＋R（P〈0.01）.Conclusion SIRT1 has an anti-fibrosis effect in rats with AKI.The possible mechanism may be through reducing the percentage of abnormally increased cells at G2/M phase.

关 键 词：沉默信息调节因子1 白藜芦醇 急性肾损伤 

分 类 号：R692[医药卫生—泌尿科学]