奶牛阴道分泌物中枯草芽孢杆菌的分离鉴定及抑菌效果分析  

Isolation and identification of Bacillus subtilis in the vaginal secretion of dairy and its antibacterial effect

作　　者：郑伟萍[1] 何剑斌[1] 李鹏[1] 龙淼[1] 张彤[1] 陈帅[1] 姜丽莹[1] 杨淑华[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110866

出　　处：《畜牧与兽医》2017年第6期174-178,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家星火计划重大项目(项目编号:2011GA650001)

摘　　要：从奶牛阴道分泌物中分离出一株细菌,经过菌落形态特征、生理生化鉴定初步判定为芽孢杆菌属;根据16S rRNA基因序列及其同源性分析结果,证实分离得到的Y4菌株是枯草芽孢杆菌,与GenBank上的枯草芽孢杆菌16S rRNA基因序列同源性为99.6%。对此菌株进行抑菌试验,发现该菌株对大肠杆菌、金黄色葡萄球菌和链球菌有较好的抑菌效果。该研究为将来研制益生菌制剂防治奶牛生殖道疾病提供了物质基础。One bacterial strain was isolated from the vaginal secretion of dairy and determined to be Bacillus based on morphological observation,physiological and biochemistry reactions. Further the isolate Y4 was identified to be Bacillus subtilis according to 16 S rRNA gene sequencing and homology analysis. The identity of 16 S rRNA gene sequence is up to 99. 6% with that of B. subtilis published on GenBank. The antibacterial assay showed that Y4 has better antibacterial effect for Escherichia coli,Staphylococcus aureus and Streptococcus. This experiment provides the foundation for devlopment of the probiotics used for treatment of genital tract diseases in dairy.

关 键 词：枯草芽孢杆菌 分离 鉴定 抑菌效果 

分 类 号：S857[农业科学—临床兽医学]