海燕皂苷P1的分离及其海燕皂苷的制备  

The isolation of asterosaponin P1 and preparation of saponin from asterinapectinifera

作　　者：胡晓娟[1] 陈淼[1] 蔡飞[1] 李峰[1] 宁寰宇[1] 郭丽霞[1] 李伟[2] 谭成玉[1] HU Xiao-juan CHEN Miao CAI Fei LI Feng NING Huan-yu GUOLi-xia LI Wei TAN Cheng-yu(College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学海洋科技与环境学院,辽宁大连116023 [2]大连海洋大学食品科学与工程学院,辽宁大连116023

出　　处：《精细与专用化学品》2017年第6期27-31,共5页Fine and Specialty Chemicals

基　　金：国家自然科学基金项目(41306137);辽宁省高校杰出青年学者成长计划(LJQ 2014078);大连海洋大学大学生创新创业项目

摘　　要：对海燕皂苷P1的分离纯化及海燕总皂苷的制备方法进行了研究。海燕经70%乙醇进行提取,石油醚、乙酸乙酯、正丁醇梯度萃取,经多种柱色谱对海星正丁醇层部分进行分离纯化,结合理化常数的测定和核磁共振光谱,鉴定了化合物结构为海燕皂苷P1(5’-O-硫酸酯24(-3-O-甲基-L-阿拉伯呋喃糖基)-5-胆甾-3,6,8,15-24-五醇),并采用闪式萃取结合热回流提取法制备海燕总皂苷,其适宜提取条件为以甲醇作为溶剂,闪式破碎5min,甲醇浓度100%,料液比1∶50(即1g海燕加入50mL提取液),加热回流时间1.5h。The isolation of asterosaponin P1 and the preparation condition of total saponins from asterinapectiniferawere studied.Asterinapectiniferawas extracted with 70% ethanol and then was extracted with petroleum ether,ethyl acetate and n-butanol.The n-buthanol fraction was isolated and identified on the basic of analysis of NMR data,the structure was identified as asterosaponin P1（5＇-O-sulfate 24（-3-O-methyl-L-arabinofuranosyl）-5-cholestane-3,6,8,15-24-pentol）.In addition,the extraction of total saponins was obtained by flash extraction combined with hot reflux method and the optimal conditions were as follows：methanol as solvent,flash extraction time was 5min,methanol concentration was 100%,the ratio of material to liquid was 1∶50（g/mL）,hot reflux time was 1.5h.

关 键 词：海燕 海燕皂苷P1 总皂苷 闪式萃取结合热回流 

分 类 号：TQ464.3[化学工程—制药化工]