不同强度电针治疗对血管性痴呆大鼠学习记忆功能及海马CA1区β淀粉样蛋白1-40表达的影响  

Effects of electroacupuncture treatment of different intensities on learning-memory function and expression of β-amyloid protein 1-40 in hippocampus CA1 region in rats with vascular dementia

作　　者：张拯[1] 杨娟娇 孙琪[1] 邵瑛[1] Zhang Zheng Yang Juanjiao Sun Qi Shao Ying(Clinical Medical College of Acupuncture and Moxibustion Rehabilitation, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China)

机构地区：[1]广州中医药大学针灸康复临床医学院,510405

出　　处：《中国脑血管病杂志》2017年第6期321-325,331,共6页Chinese Journal of Cerebrovascular Diseases

摘　　要：目的观测不同强度电针治疗对血管性痴呆大鼠学习记忆功能及海马CA1区β淀粉样蛋白1-40(Aβ1-40)表达的影响,寻求最佳电针治疗强度。方法共纳入60只雄性SPF级SD大鼠,采用随机数字表法随机选取8只大鼠为假手术组,其余大鼠采用改良的四血管阻断(4-VO)法复制VD模型,将造模成功的大鼠(24只)按随机数字表法完全随机分为模型组、1 m A电针组(频率2/15 Hz,强度1 m A,留针时间20 min)、3 m A电针组(频率2/15 Hz,强度3 m A,留针时间20 min),每组8只。电针组针刺"百会"、"大椎"穴,1次/d,连续治疗10 d,休息2 d为1个疗程。2个疗程后,采用Morris水迷宫试验检测各组大鼠学习记忆能力,运用荧光定量聚合酶链反应法检测大鼠海马CA1区Aβ1-40 m RNA表达水平。结果假手术组、模型组、1 m A电针组、3 m A电针组大鼠Morris水迷宫试验第2~5天平均逃避潜伏期分别为(46.8±1.9)、(40.6±2.3)、(24.6±1.5)、(19.4±1.2)s;(56.3±3.5)、(51.2±2.6)、(45.9±2.1)、(40.8±1.4)s;(52.7±1.5)、(46.0±2.3)、(31.3±1.2)、(27.7±1.6)s;(50.8±3.9)、(41.5±2.1)、(29.0±1.1)、(25.6±1.3)s;首次跨越原平台时间分别为(23.3±1.6)、(53.9±1.3)、(30.2±1.4)、(28.1±0.8)s,120 s内跨越原平台次数分别为(9.4±0.9)、(2.6±0.5)、(6.4±0.7)、(7.2±0.9)次;CA1区中Aβ1-40 mRNA表达水平分别为(17.3±1.1)、(40.7±1.1)、(24.0±1.7)、(22.4±1.8),组间差异均有统计学意义(F值分别为195.88、861.605、103.876、380.609,均P<0.01);1 m A、3 m A电针组大鼠平均逃避潜伏期、首次跨越原平台时间较模型组明显缩短,120 s内跨越原平台次数较模型组明显增加,CA1区中Aβ1-40 mRNA表达水平较模型组明显降低,且3 m A电针组均明显优于1 m A电针组,差异均有统计学意义(均P<0.05)。结论电针治疗可改善VD大鼠学习记忆能力并降低海马CA1区Aβ1-4 0 mRNA表达水平,3 m A电针效果优于1 m A电针。Objectives To observe the effects of electroacupuncture （EA） treatment of different intensities on learning-memory function and expression of β-amyloid protein 1-40 （Aβ1-40） in hippocam- pus CA1 region in rats with vascular dementia （VD） and to seek the best intensity of EA treatment. Methods A total of 60 male SD rats （SPF grade） were enrolled. Eight rats were selected as sham operation group with random number table method;others were used to copy the VD model with the modified four-vessel occlusion （4-VO） method. According to the random number table method, the successful model of the rats （n = 24） were completely randomly divided into a model group, a 1 am EA group （frequency, 2/15 Hz, intensity, I mA, needle retention time, 20 min ） , and a 3 mA EA group （ frequeney, 2/15 Hz, intensity, 3 mA, needle retention time,2Omin;n =8 in each group）. DU20 （BaiHui） and DU14 （DaZhui） in the EA group were acupunctured once a day for 10d,and took a rest for 2d as 1 treatment course. After 2 treatment courses, Morris water maze test was used to detect the ability of learning and memory of rats in each group. Fluorescent quantitative polymerase chain reaction was used to detect the expression level of Aβ1 40 mRNA. Results The mean escape latencies of the water maze test from 2 to 5 days in the sham operation group, model group, 1 mA E A group, and 3 mA EA group were 46.8 ± 1.9,40.6 ± 2.3,24.6 ± 1.5,19.4 ± 1.2 s;56.3 ±3.5,51.2±2.6,45.9 ±2.1,40.8 ±1.4 s,52.7 ±1.5,46.0±2.3,31.3 ± 1.2,27.7 ± 1.6s; and 50.8±3.9,41.5 ± 2.1,29.0 ± 1.1,25.6 ± 1.3 s, respectively; the first time across the original platform were 23.3 ± 1.6,53.9±1.3,30.2 ± l. 4,and 28.1 ±0.8 s, respectively;the first across the original plat- form within 120 s were 9.4 ± 0.9,2.6 ± 0.5,6.4 ± 0.7, and 7.2 ±0.9, respectively ; the expression levels of Aβ 1-40 mRNAs in the CA1 regions were 17.3 ± 1.1,40.7 ± 1.1,24.0 ± 1.7 ,and 22.4 ± 1.8 ,respec- tively. There were significant differences among the g

关 键 词：血管性痴呆 电针强度 学习记忆能力 β淀粉样蛋白140 

分 类 号：R245[医药卫生—针灸推拿学]