‘火焰’品种彩色马蹄莲组培快繁体系的建立及优化  

The Establishment and Optimization of Tissue Culture and Rapid Propagation System of Zantedeschia hybrida 'Flame'

作　　者：张慧 罗珍珍 由翠荣[2] 孔艳辉 解晓旭 孙印兵 

机构地区：[1]烟台市园林管理处科研所,山东烟台264000 [2]烟台大学生命科学学院,山东烟台264005

出　　处：《安徽农业科学》2017年第16期133-135,146,共4页Journal of Anhui Agricultural Sciences

基　　金：山东省科技发展计划项目(2013GNC11037);烟台市科技发展计划项目(2015GNC113)

摘　　要：[目的]建立并优化‘火焰’品种彩色马蹄莲组培快繁体系。[方法]以‘火焰’品种彩色马蹄莲的球茎嫩芽为试验材料,对其组织培养无性繁殖体系进行研究。[结果]最佳诱导培养基为MS+2.0 mg/L 6-BA+0.2 mg/LNAA;最佳增殖培养基为MS+2.0 mg/L6-BA+0.2 mg/L NAA;植株再生最适合培养基为1/2MS+0.2 mg/L 6-BA+0.2 mg/L NAA;最适生根培养基为1/2MS+0.2 mg/L NAA+2.0 g/L活性炭。[结论]该研究建立了‘火焰’品种彩色马蹄莲的组培快繁体系,为其快速繁殖及产业化生产提供了技术和理论依据。[ Objective ] To establish and optimze the tissue culture and rapiad progration system of Zantedeschia hybrida ＇ Flame＇. [ Method ] The bulb shoots of Z. hybrida ＇ Flame＇ were used as experimental materials to study the system of tissue culture and rapid propagation. [ Result ] The optimal medium for induction was MS ＋ 2.0 mg/L 6 - BA ＋ 0.2 mg/L NAA. The best proliferation medium was MS ＋ 2.0 mg/L 6 - BA ＋ 0.2 mg/L NAA. The most suitable plant regeneration medium was 1/2MS ＋ 0.2 mg/L 6 - BA ＋ 0.2 mg/L NAA. The optimum rooting medium was 1/2MS ＋ 0.2 mg/L NAA ＋ 2.0 g/L activated carbon. [ Conctustion ] The tissue culture and rapid propagation system of Z. hybrida＇ Flame＇ was established to provide technology and the theoretical fomdation for its rapid propagation and industrialized production.

关 键 词：'火焰’彩色马蹄莲 组培快繁 优化 

分 类 号：S682.264[农业科学—观赏园艺]