MMP-1、7、9基因多态性与青海回、汉族胎膜早破相关性  

The correlation study between MMP-1,7,9 gene polymorphism and premature rupture of membranes in the pregnant woman in Qinghai Han-and Hui-Chinese

作　　者：白玉芳[1] 祁存秀 路丽[2] 沙仁高娃[2] 王荣华[3] 朱雪玲[2] 巩海风[2] 苏占海[4] BAI Yu-fang QI Cun-xiu LU Li SHAREN-Gaowa WANG Rong-hua ZHU Xue-ling GONG Hai-feng SU Zhan-hai(Dept. Of Obstetrics, Qinghai University Affiliated Hospital Graduate School of Qinghai University Supply Room, Qinghai University Affiliated Hospital Basic Medical Research Center, Qinghai University Medical College,Xining, Qinghai, 810001)

机构地区：[1]青海大学附属医院产科 [2]青海大学研究生院 [3]青海大学附属医院供应室 [4]青海大学医学院基础医学研究中心,西宁青海810001

出　　处：《中国高原医学与生物学杂志》2017年第2期103-108,共6页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省应用基础研究项目(2016-ZJ-762);青海省卫生计生委指导性课题(2015)

摘　　要：目的利用PCR及测序方法分析青海地区回、汉族胎膜早破孕妇MMP-1、7、9基因多态性特点及是否具有民族差异性。方法提取胎膜早破孕妇和对照组组织DNA,利用PCR和琼脂糖凝胶电泳、测序法,检测基因多态性并行相关分析。结果 MMP-1 rs1799750 G/G、-/G、-/-基因型频率在汉族对照组和胎膜早破组中分别是44.25%、51.33%、4.42%和34.29%、47.14%、18.57%,两组基因型频率分布具有显著差异性(P=0.006);在回族对照组和胎膜早破组中基因型频率分别是45.98%、50.57%、3.45%和51.72%、34.48%、13.79%,两组基因型频率呈现显著差异性(P=0.005)。MMP-7 rs11568818三种基因型A/A、A/G、G/G在汉族对照组和胎膜早破组中基因型频率分别是60.18%、37.17%、2.65%和84.29%、8.57%、7.14%,两组相比,基因型频率分布具有显著差异性(P=0.000);回族对照组和胎膜早破基因型频率分布分别是9.77%、39.08%、1.15%和86.21%、6.90%、6.90%,两组基因型频率相比具有显著差异性(P=0.000)。MMP-9 rs3918242 SNP三种基因型C/C、C/T、T/T在汉族对照组和胎膜早破组中基因型频率分布分别是72.57%、26.55%、0.88%和70.00%、28.57%、1.43%,其基因型频率在两组中无显著性差异(P=0.895);在回族对照组和胎膜早破组中基因型频率分别是71.26%、8.74%、0.00%和70.69%、27.59%、1.72%,两组相比无显著差异性(P=0.468)。结论 MMP-1 rs1799750和MMP-7 rs11568818基因多态性可能与青海汉、回族胎膜早破相关;而MMP-9 rs3918242基因多态性与胎膜早破无关。同时,MMP-1rs1799750、MMP-7 rs11568818和MMP-9 rs3918242基因频率分布在青海汉、回族对照组和胎膜早破组中无特异性。Objective To analyze the polymorphism of MMP-1,7,9 gene in pregnant women with premature rupture of membranes in Han- and Hui-Chinese at Qinghai province by PCR and sequencing.Methods Genome DNA was extracted from preterm premature rupture of membranes and control group.The polymorphism of the gene was analyzed by PCR, agarose gel electrophoresis and sequencing. Results The frequency of MMP-1 rs1799750 G/G, -/G, -/- genotype was 44.25%, 51.33%, 4.42% and 34.29%, 47.14%, 18.57%, respectively, in the Han control and the premature rupture of membranes groups, the genotype frequency was significantly different between two groups（ P = 0.006）.Meanwhile the frequency of genotype was 45.98%, 50.57%, 3.45% and 51.72%, 34.48%, 13.79% respectively in the control and the premature rupture of membranes group in Hui Chinese, and the genotype frequency was obviously different between two groups （ P = 0.005 ）. The frequency of MMP- 7 rs11568818 A/A, A/G,G/G genotype was 60.18%,37.17%,2.65% and 84.29%,8.57% ,7.14% in the Han control and the prema- ture rupture of membranes groups, the genotype frequency was significantly different between two groups （P=0.000） .Meanwhile the frequency of genotype was 9.77%, 39.08%, 1.15% and 86.21%, 6.90%, 6.90%, re- spectively, in the control and the premature rupture of membranes group in Hui Chinese, and the genotype frequency was obviously different between two groups （ P = 0.000）.The frequency of MMP-9 rs3918242 C/C, C/T, T/T geno- type was 72.57% ,26.55% ,0.88% and 70.00% ,28.57%, 1.43% in the Han control and the premature rupture of membranes groups, the genotype frequency was significantly different between two groups （P = 0.895 ）.Meanwhile the frequency of genotype was 71.26%, 8.74%, 0.00% and 70.69%, 27.59%, 1.72%, respectively, in the control and the premature rupture of membranes group in Hui Chinese,and the genotype frequency was obviously different be- tween two groups （ P = 0.468 ）. Conclusion The polymorphisms of MMP- 1 rs 1799750 and MMP- 7 rs 11568818 may

关 键 词：MMP基因 基因多态性 胎膜早破 青海 回族 汉族 

分 类 号：R393[医药卫生—基础医学]