“桂枝与白芍”药对化学成分UPLC-Q/TOF-MS分析  

Chemical constituents analysis of the drug pair of "Cinnamomum cassia Presl and Paeoniae Radix Alba" by UPLC-Q/TOF-MS

作　　者：陈永财 钱江辉 王彬辉[4] 沙先谊[2,3] CHEN Yongcai QIAN Jianghui WANG Binhui SHA Xianyi(Department of Pharmaceutics, Hospital of TCM in Wenzhou City, Zhejiang Province, Wenzhou 525000, China Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 201203, China Institute of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai 201203, China Department of Pharmaceu- tics, Affiliated Municipal Hospital of Taizhou Medical College, Zhejiang Province, Taizhou 318002, China)

机构地区：[1]浙江省温州市中医院药剂科,浙江温州325000 [2]复旦大学药学院药剂学教研室,上海201203 [3]复旦大学中西医结合研究院,上海201203 [4]台州学院医学院附属市立医院药剂科,浙江台州318002

出　　处：《中国医药导报》2017年第16期12-15,23,共5页China Medical Herald

基　　金：浙江省中医药资助项目(2015ZA195);浙江省温州市公益性科技计划项目(Y20140746)

摘　　要：目的采用液质联用技术(UPLC-Q/TOF-MS)对"桂枝与白芍"药对中的化学成分进行定性分析。方法采用Agilent 1290 UPLC和YMC-Triart C_(18)(100 mm×2.1 mm,1.9μm)色谱柱进行快速物质分离;流动相为乙腈-0.1%冰醋酸梯度洗脱;温度:30℃;进样量:5μL;检测波长:254 nm;流速:0.3 mL/min;AB 5600质谱使用ESI离子源分别在正离子与负离子模式下采集数据。结果结合对照品确认了8个化合物,通过质谱信息与相关文献数据推测13个化学成分,对其进行成分归属。其中主要含有没食子酸、原儿茶酸等6种有机酸,芍药苷、芍药内酯苷等10种芍药总苷单萜类化合物及香豆素类成分。结论采用UPLC-Q/TOF-MS确定"桂枝与白芍"药对中的化学成分,为阐明"桂枝与白芍"药对的药效物质基础提供有力的证据。Objective To develop an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometric （UPLC-Q/TOF-MS） method to analyze the chemical constituents in the drug pair of ＂Cinnamomum cassia Presl and Paeoniae Radix Alba＂. Methods Agilent 1290 UPLC machine and YMC-Triart Cis column （100 mm × 2.1 mm, 1.9 μm） were adopted to separate ingredients; the mobile phase was acetonitrile （A）-0.1% acetic acid solution （B） with D＇adient elution; the column temperature was 30℃; the inject volume was 5 μL; the detection wavelength was set at 254 nm; the flow rate was 0.3 mL/min; the mass spectrometer AB 5600 with an electrospray ionization （ESI） interface was under positive or negative mode for data collection. Results 8 compounds were identified by reference substance, 13 compounds were qualitative identified according to the MS/MS information, and the belonging of the main constituents had been confirmed. These compounds included six kinds of organic acid such as gallic acid and protocatechuic acid, tentotal paeoniflorin monoterpenes like paeoniflorin and albiflorin, and coumarins. Conclusion Determination of chemi- cal composition of ＂Cinnamomum cassia Presl and Paeoniae Radix Alba＂ by UPLC-Q/TOF-MS in order to clarify the ＂Cinnamomum cassia Presl and Paeoniae Radix A lba＂ drug on the basis of the material to provide strong evidence.

关 键 词：桂枝 白芍 药对 UPLC-Q/TOF-MS 成分鉴定 物质基础 

分 类 号：R927[医药卫生—药学]