民族药刺齿凤尾蕨抑制人源结肠癌细胞活性成分研究  

Study on chemical constituents and their inhibition of anthropogenic colon cancer cells bioactivity from ethnic medicine Pteris dispar

作　　者：孙丹[1] 张艳[1] 孙强[1] 彭玉勃 王磊[1] SUN Dan ZHANG Yan SUN Qiang PENG Yubo WANG Lei(Jiamusi College, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China)

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中国医药导报》2017年第16期20-23,共4页China Medical Herald

摘　　要：目的研究民族药刺齿凤尾蕨抑制人源结肠癌细胞活性成分。方法采用95%乙醇提取药材,聚酰胺色谱、葡聚糖凝胶色谱以及制备型高效液相色谱等技术获取化合物,磁共振波谱技术(NMR)对得到的化合物进行结构鉴定。磺酰罗丹明B比色法(SRB)对化合物进行体外抗肿瘤活性筛选。结果从刺齿凤尾蕨中分离得到5个二萜类化合物,分别鉴定为geopyxin A(1),geopyxin C(2),ent-11β-acetoxykaur-16-en-18-ol(3),7β,16α,17-trihydroxy-ent-kauran-19-oic acid(4)和neolaxiflorin K(5)。活性筛选表明化合物1,2和5具有显著的抑制人源结肠癌细胞活性,IC50值分别为1.20、2.45和3.26μmol/L。结论化合物1~5首次从刺齿凤尾蕨中分离得到。Objective To study the inhibition of anthropogenic colon cancer cells activity constituents of Pteris dispar. Methods The medicinal materials were extracted by 95% ETOH. Compounds were obtained by kinds of chromatographies including polyamide, Sephadex LH-20 and pre-HPLC and the structures identified by NMR. In vitro antitumor activity was tested by SRB methods. Results 5 compounds were obtained from the plant and their structures were established as geopyxin A （1）, geopyxin C （2）, ent-11β-acetoxykaur-16-en-18--ol （3）, 7β, 16α, 17-tribydroxy-ent-kauran-19-oic acid （4） and neolaxiflorin K （5）. Compounds 1, 2 and 5 showed strong in vitro antitumor activity against HCT-116, with IC50 values of 1.20, 2.45 and 3.26 μmol/L respectively. Conchlsion Compounds 1-5 are obtained from Pteris dispar for the first time.

关 键 词：刺齿凤尾蕨 化学成分 体外抗肿瘤活性 

分 类 号：R927[医药卫生—药学]