胆固醇性肺炎中TLR4、MyD88和NF-κB p65的表达及意义  

Expression and significance of TLR4,MyD 88 and NF-κB p65 in cholesterol pneumonia

作　　者：康丽菲[1] 邸红芹[2] 朱桂云[1] 安晓颖[1] 陈宁[1] 杨永辉[1] 

机构地区：[1]河北省胸科医院病理科,河北石家庄050041 [2]河北省胸科医院检验科,河北石家庄050041

出　　处：《河北医科大学学报》2017年第6期680-684,共5页Journal of Hebei Medical University

基　　金：河北省医学科学研究重点课题(ZL20140205)

摘　　要：目的检测Toll样受体4(Toll like receptor 4,TLR4)、髓样分化因子88(myeloid differentiation factor88,MyD88)和核因子-κB p65(nuclearfactor-kappaB p65,NF-κB p65)在胆固醇性肺炎泡沫细胞中的表达水平,探讨三者在胆固醇性肺炎发生中的作用、相互关系及临床意义。方法选择手术切除标本经病理证实为胆固醇性肺炎45例,普通型肺炎(对照组)25例,采用免疫组织化学方法检测2组TLR4、MyD88和NF-κB p65的表达水平。结果胆固醇肺炎泡沫细胞中TLR4蛋白阳性表达率、MyD88蛋白和NF-κB p65蛋白强阳性表达率明显高于对照组,差异有统计学意义(P<0.05)。TLR4阳性表达率、MyD88和NF-κB强阳性表达率均与性别不相关(P>0.05)。年龄<50岁者胆固醇肺炎泡沫细胞中TLR4阳性表达率、MyD88和NF-κB p65强阳性表达率均明显高于年龄≥50岁者,差异有统计学意义(P<0.05)。TLR4与MyD88、TLR4与NF-κB p65、NF-κB p65与MyD88在胆固醇性肺炎的表达中均呈明显正相关(r=0.476、0.523、0.491,P<0.05)。结论 TLR4、MyD88和NF-κB p65的阳性表达可能与胆固醇性肺炎的发生发展有关,三者可能在胆固醇性肺炎的发病中起协同作用。随着年龄增长,三者表达的阳性率均降低。Objective To detect the expression of Toll-like receptor 4（TLR4）,Myeloid differentiation factor 88（MyD88）and nuclear factor-κB p65（NF-κB p65） in cholesterol pneumonia foam cells,and to investigate their roles and relationship in the occurrence of cholesterol pneumonia and clinical significance.Methods The expression of TLR4,MyD88 and NF-κB p65 in 45 cases of foam cells of cholesterol pneumonia and 25 cases of macrophages of usual pneumonia without the aggregation of foam cells（control groups） from surgically resecting specimens of lung that was confirmed by pathology was immunohistochemically studied.Results The strong positive rate of MyD88 and NF-κB p65,as well as the positive rate of TLR4,was obviously higher than that in the control（P〈0.05） The positive expression of TLR4,MyD88 and NF-κB p65 was not related with sex（P〉0.05）.The expression rate of TLR4,MyD88 and NF-κB p65 in patience below 50 was more significant higher than the patience above 50（P〈0.05）.Among the 45 cases of cholesterol pneumonia,there were apparently positive correlation between TLR4 and MyD88,TLR4 and NF-κB,NF-κB p65 and MyD88（r=0.467,0.523,0.491,P〈0.05）,as well as the positive correlation.Conclusion The expression of TLR4,MyD88 and NF-κB p65 may be involved in pathogenesis of cholesterol pneumonia presenting their synergistic effect in cholesterol pneumonia,which was not related to the sex but age.With the increase in age of the cholesterol pneumonia,the positive rate of TLR4,MyD88 and NF-κB p65 was decreased.

关 键 词：胆固醇性肺炎 TOLL样受体4 髓样分化因子88 NF-ΚB 

分 类 号：R563.1[医药卫生—呼吸系统]