SerpinB2和SerpinB4基因在过敏性鼻炎患者鼻刷细胞的表达  

Expression of SerpinB2 and SerpinB4 genes in nasal brushings in allergic rhinitis

作　　者：王敏[1] 佘文煜[1] 杨军[1] 王向东[1] 张旭[1] 张罗[1] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科、北京市耳鼻咽喉科研究所、耳鼻咽喉头颈科学教育部重点实验室(首都医科大学)、鼻病研究北京市重点实验室,北京100005

出　　处：《中国耳鼻咽喉头颈外科》2017年第6期312-315,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：教育部创新团队发展计划(IRT13082);国家自然科学基金重点项目(8163002)、国家自然科学基金重点国际合作研究项目(81420108009)、国家自然科学基金(81570893);首都卫生发展科研专项项目(首发2014-4-1092);北京市自然科学基金(7162042)联合资助

摘　　要：目的探讨SerpinB2和SerpinB4基因mRNA在过敏性鼻炎(AR)患者鼻刷细胞中的表达及其与鼻刷细胞嗜酸细胞数目、血清总IgE(total IgE,tIgE)和AR严重程度的关系。方法募集29例健康对照和59例AR患者,其中特异性IgE阳性AR患者(specific IgE positive AR,sIgE-P-AR)29例,特异性IgE阴性自我报告AR患者(specific IgE negative self reported AR,sIgE-N-SR-AR)30例。收集鼻刷细胞和外周血,检测鼻刷细胞SerpinB2和SerpinB4基因mRNA表达、计数嗜酸细胞数目、检测外周血tIgE和过敏原特异性IgE。结果鼻刷细胞SerpinB2和SerpinB4基因mRNA在sIgE-P-AR中的表达相对值分别为5.17(2.33~18.96)、0.6(0.355~1.08),在sIgE-N-SR-AR中的表达相对值分别为3.27(1.59~13.4)、0.75(0.42~1.64),均显著高于健康对照1.21(0.1~3.285)、0.29(0.165~0.505)(sIgE-P-AR:P<0.001,P=0.013;sIgE-N-SR-AR:P=0.002,P<0.001)。AR患者鼻刷细胞SerpinB2和SerpinB4基因mRNA表达与鼻刷细胞嗜酸细胞数目和tIgE无相关性。SerpinB2在中重度AR患者中的表达相对值为4.74(2.68~47.5),显著高于轻度AR患者2.495(1.333~5.603)(P=0.025),而SerpinB4基因在轻度AR患者中的表达相对值为3.95(2.6~7.59),显著高于中重度AR患者2.83(0.715~5)(P=0.042)。结论 SerpinB2和SerpinB4基因可能参与了AR的发生,可作为候选基因后续进一步扩大样本量评价其作为AR诊断指标的效果。OBJECTIVE To investigate the expression of SerpinB2 and SerpinB4 genes in cells from the nasal brushings in allergic rhinitis（AR） patients, and their relationships with eosinophil numbers in nasal brushings, serum total IgE level and severity of AR. METHODS Twenty nine control subjects and 59 AR patients[29 specific IgE positive AR（sIgE-P-AR） patients, 30 specific IgE negative self reported AR（sIgE-N-SR- AR） patients] were recruited. The samples of nasal brushings and peripheral blood were collected to detect SerpinB2 and SerpinB4 gene expression and eosinophil numbers in nasal brushings, and total IgE and allergen-specific IgE level in peripheral blood. RESULTS Expression of SerpinB2 and SerpinB4 genes in cells from nasal brushings were significantly higher in slgE-P-AR [5.17（2.33-18.96）, 0.6（0.355-1.08）, respectively] and sIgE-N-SR-AR [3.27（1.59-13.4）, 0.75（0.42-1.64）, respectively] than that in control subjects[1.21（0.1-3.285）, 0.29（0.165-0.505）] （sIgE-P-AR： P=0.013; sIgE-N-SR-AR： P=0.002）. Expression level of SerpinB2 and SerpinB4 had no relationships with eosinophil numbers in nasal brushings and serum total IgE level. Expression of SerpinB2 in moderate/ severe AR[4.74（2.68-47.5）] was significantly higher than that in mild AR[（1.333-5.603）]（P=0.025）; while expression of SerpinB4 in mild AR[3.95（2.6-7.59）] was significantly higher than that in moderate/severe AR[2.83（0.715-5）]（P=0.042）. CONCLUSION SerpinB2 and SerpinB4 genes might be involved in the pathogenesis of AR, and their diagnostic values in AR deserve to be evaluated with larger samples.

关 键 词：鼻炎 变应性 常年性 鼻炎 变应性 季节性 聚合酶链反应 鼻刷细胞 SerpinB2 SerpinB4 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]