HMGB1在脓毒症心肌损害小鼠中的表达及炎症促进作用研究  

Expression of HMGB1 in sepsis myocardial injury and its role in promoting inflammation

作　　者：冯宣云 艾山木[1] 刘琼[1] Feng Xuanyun Ai Shanmu Liu Qiong(Department of Emergency Medicine ＆ Critical Care Medicine,The First Affiliated Hospital of Chongqing Medical Universit)

机构地区：[1]重庆医科大学附属第一医院急诊医学科&重症医学科,重庆400016

出　　处：《重庆医科大学学报》2017年第5期509-514,共6页Journal of Chongqing Medical University

摘　　要：目的:探究高迁移率族蛋白B1(high mobility group box-1 protein,HMGB1)在脓毒症心肌损害(sepsis induced myocardial depression,SIMD)中的表达以及通过HMGB1抑制剂甘草酸(glycyrrhizin,GL)在SIMD中的作用。方法:将小鼠分为正常组、盲肠结扎穿孔(cecal ligation and puncture,CLP)模型组、CLP+PBS对照组和CLP+GL干预组,每组随机分配5只C57BL/6雄性小鼠,通过ELISA检测小鼠血清、心脏匀浆液中高迁移率族蛋白B1(high mobility group box-1 protein,HMGB1)的表达,RT-PCR对小鼠心肌组织中HMGB1进行定量分析,使用GL干预CLP小鼠模型后检测外周血中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-1β(interleukin-1β,IL-1β)、IL-6、IL-10、IL-33等细胞因子水平变化。结果:(1)CLP诱导SIMD小鼠模型中,HMGB1以及心肌损害标志物c TnⅠ的表达较正常对照组明显升高(P<0.05);(2)GL处理SIMD小鼠模型后,TNF-α、IL-1β、IL-6、IL-10、IL-33等细胞因子以及心肌损害标志物c TnⅠ表达较对照组和模型组明显降低(P<0.01)。结论:抑制HMGB1可降低机体各种炎症因子水平,调控脓毒症的失控性炎症效应,减轻脓毒症心肌损害。Objective：To explore expression of high mobility group box-1 protein（HMGB1）in sepsis induced myocardial depression（SIMD）and the role of inhibiting glycyrrhizic（GL）in SIMD. Methods：Mice were divided into normal group,cecal ligation and perforation（cecal ligation and puncture,CLP）model group,CLP＋PBS control group and CLP＋GL group,each group were randomly assigned to 5 male C57BL/6 mice. Expressions of HMGB1 ELISA in serum,heart homogenate,RT-PCR of HMGB1 in myocardial tissue of mice were detected by quantitative analysis of changes the factor level in peripheral blood of tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β）,IL-6,IL-10 and IL-33 cells were detected by GL CLP mice after intervention. Results：CLP induced mouse model of SIMD. HMGB1 and myocardial damage marker expression in c TnⅠ was obviously higher than that of normal control group（P〈0.05）. SIMD mice model of GL after treatment,TNF-α,IL-1β,IL-6,IL-10,IL-33 cytokines,myocardial damage marker c Tn I expression was significantly lower than that of control group and the model group（P〈0.01）. Conclusion：Inhibiting HMGB1 can reduce the level of inflammatory factors in the body,control the uncontrolled inflammatory effect of sepsis,and reduce the myocardial damage in sepsis.

关 键 词：高迁移率族蛋白B1 脓毒症心肌损害 炎症因子 

分 类 号：R542.2[医药卫生—心血管疾病]