云南大理某农村猪星状病毒分子流行病学调查  

Molecular epidemiology of porcine astrovirus in rural area of Dali City,Yunnan Province,China

作　　者：刘承波[1,2] 宿凌云[2] 邹莉[2] 杨琳娜[2] 董薇[2] 马倩 闫雪兰[2] 冯云[2] LIU Cheng-bo SU Ling-yun ZOU Li YANG Lin-na DONG Wei MA Qian YAN Xue-lan FENG Yun(Medical Faculty, Kunming University of Science and Technology, Kunming 650500, Yunnan Province, Chin)

机构地区：[1]云南昆明理工大学医学院,云南昆明650500 [2]云南省第一人民医院生殖妇科,云南昆明650032

出　　处：《中国生物制品学杂志》2017年第6期596-599,606,共5页Chinese Journal of Biologicals

基　　金：云南省卫生科技计划项目(2014NS234;2014NS233;2014-NS365;2014NS366);云南省科技惠民谋划(2014RA019)

摘　　要：目的了解云南大理某农村猪群中星状病毒(astrovirus,AstV)感染情况,为AstV的防控提供理论依据。方法利用逆转录巢式聚合酶链反应(RT-nested PCR)技术,对云南大理某农村采集的42份猪粪便样品进行PAstV799 bp基因部分片段扩增、克隆,并测序;利用NCBI BLAST进行数据库比对,Mega 4.1软件进行序列同源性比较和系统进化树构建。结果 Trizol试剂提取42份猪粪便样品上清总RNA,经RT-nested PCR扩增,5份样品可见799 bp的目的基因片段,阳性率为11.90%;PAstV 799 bp片段同源性分析显示,5个毒株与PAstV-1、2、3、4和5的同源性为46.3%~87.9%,与PAstV-4的同源性最高,为75.5%~87.9%;PAstV 799 bp片段的系统进化分析显示,分离的毒株属于PAstV-4,同源性为80.0%~99.5%。结论云南省PAstV-4流行病学调查将有利于中国AstV的预防及治疗。Objective To investigate the prevalence of porcine astrovirus（Ast V） infection in rural area of Dali City,Yunnan Province, China, and provide a theoretical basis for the prevention and control of Ast V. Methods A 799 bp PAst V gene fragment was amplified from 42 swine stool samples by reverse transcription-nested polymerase chain reaction（RT-nested PCR）, then cloned, sequenced, searched in NCBI BLAST data, and analyzed for homology by Mega 4. 1software, based on which a phylogenetic tree was constructed. Results Total RNA was extracted from the supernatant of42 swine stool samples by using Trizol reagent, with 5 of which the target gene was amplified by RT-nested PCR,indicating a positive rate of 11. 90%. The homologies of five strains to PAst V-1, 2, 3, 4 and 5 were 46. 3% ~ 87. 9%,while those to PAst V-4 were the highest, which were 75. 5% ~ 87. 9%. Phylogenetic analysis showed that the isolated strains belonged to PAst V-4, of which the homologies were 80. 0% ~ 99. 5%. Conclusion The epidemiological investigation on PAst V-4 in Yunnan Province will be beneficial to the prevention and treatment of Ast V in China.

关 键 词：猪星状病毒 逆转录巢式聚合酶链反应 流行病学 

分 类 号：S852.659.6[农业科学—基础兽医学]