NIRF蛋白对妊娠早期绒毛滋养细胞增殖、凋亡的影响及其作用机制  

Effect of NIRF on proliferation and apoptosis of early pregnancy trophoblast cells and relevant mechanism

作　　者：钱冠华[1] 董晓静[1] 于廷和[1] 段昌柱[2] QIAN Guan-hua DONG Xiao-jing YU Ting-he DUAN Chang-zhu(Department of Gynaecology and Obstetrics, The Second Hospital Affiliated to Chongqing Medical University, Chongqing 400010, China)

机构地区：[1]重庆医科大学附属第二医院妇产科,重庆400010 [2]重庆医科大学细胞生物学及遗传学教研室,重庆400016

出　　处：《中国生物制品学杂志》2017年第6期613-617,622,共6页Chinese Journal of Biologicals

基　　金：重庆市科委资助项目(cstc2014jcyj A10066)

摘　　要：目的观察NIRF蛋白对妊娠早期绒毛滋养细胞增殖、凋亡的影响及其作用机制。方法取正常妊娠妇女人工流产绒毛组织(8~10周),按常规分离方法获得滋养细胞,进行原代培养。通过上/下调NIRF在滋养细胞中的表达后,采用qRT-PCR法检测p53基因mRNA的转录水平;Western blot法检测p53蛋白的表达水平;MTT法检测滋养细胞的增殖能力;Annexin V/PI双染色法检测细胞凋亡情况。结果与未转染组比较,NIRF上/下调后,p53基因m RNA转录水平差异无统计学意义(P>0.05);NIRF上调,p53蛋白表达水平明显下降(P<0.05),早期绒毛滋养细胞增殖能力显著增强(P<0.05),凋亡率差异无统计学意义(P>0.05);NIRF下调,p53蛋白的表达水平明显升高(P<0.05),早期绒毛滋养细胞增殖能力明显减弱(P<0.05),凋亡率明显上升(P<0.05)。结论 NIRF在蛋白水平上抑制了p53的表达,促进了滋养细胞的增殖。Objective To investigate the effect of NIRF on the proliferation and apoptosis of early pregnancy trophoblast cells as well as the relevant mechanism. Methods Early pregnancy trophoblast cells were isolated by conventional methods from the villi（8 to 10 weeks） of normal pregnant women having artificial abortion, and subjected to primary culture. After up/down regulation of NIRF expression, the m RNA transcription levels of p53 genes in the cells were determined by q RT-PCR, while the expression levels of p53 by Western blot. The proliferation of early pregnancy trophoblast cells was determined by MTT assay, while the apoptosis by Annexin V/PI staining. Results The transcription level of p53 m RNA after up/down regulation of NIRF expression showed no significant difference with that in untransfected cells（P〈0. 05）. After the expression of NIRF was up-regulated, the expression level of p53 deceased significantly（P〈0. 05）, the proliferation level of early pregnancy trophoblast cells increased significantly（P〈0. 05）,while the apoptosis rate showed no significant change（P〈0. 05）. However, after the expression of NIRF was downregulated, the expression level of p53 increased significantly（P〈0. 05）, the proliferation level decreased significantly（P〈0. 05）, and the apoptosis rate increased significantly（P〈0. 05）. Conclusion NIRF inhibited the expression of p53 at protein level and promoted the proliferation of trophoblast cells.

关 键 词：NIRF蛋白 P53蛋白 滋养细胞 细胞增殖 细胞凋亡 

分 类 号：Q26[生物学—细胞生物学]