基于液相色谱-质谱的雄激素检测及对干燥综合征的诊疗价值  

LC-MS based measurement of androgens and its clinical significance in diagnosis of Sj（o）gren＇s syndrome

作　　者：童海江[1] 童鸿斌 王新昌[3] 李晓芳[4] 叶剑 许志良[5] Tong Haijiang Tong Hongbin Wang Xinchang Li Xiaofang Ye Jian Xu Zhiliang(Department of Clinical Laboratory, the Second Hospital of Shaoxing , Shaoxing , Zhejiang 312000, China （ Tong H J Health - Bank Medical Laboratory Co. Ltd. , Hangzhou, Zhejiang 310052, China （ Tong liB, Ye J Department of Rheumatology and Immunology, the Second Hospital Affiliated to the Traditonal Chinese Medical University of Zhejiang, Hangzhou, Zhejiang 310005, China （ Wang XC Department of Clinical Laboratory, the Traditonal Chinese Medicine Hospital of Zhejiang Province, Hangzhou , Zhejiang 310006, China （ Li XF College of Basic Medicine, the Traditonal Chinese Medical University of Zhejiang ,Hangzhou ,Zhejiang 310053, China （ Xu ZL)

机构地区：[1]绍兴第二医院检验科,浙江省绍兴312000 [2]杭州汉库医学检验所有限公司,浙江省杭州310052 [3]浙江中医药大学附属第二医院风湿免疫科,浙江省杭州310005 [4]浙江省中医院检验科,浙江省杭州310006 [5]浙江中医药大学基础医学院,浙江省杭州310053

出　　处：《中国基层医药》2017年第13期1950-1953,I0004,共5页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省医药卫生科技计划项目（2014KYB285）

摘　　要：目的 探讨运用液相色谱-质谱（LC-MS）检测血清中的6种雄激素水平对干燥综合征（Sj（o）gren′s syndrome,SS）的诊断价值.方法 选择32例SS患者（SS组）及体检的25例体检者（健康组）.采集的血清样本经处理后,运用LC-MS进行6种雄激素水平分析.实验数据处理使用主成分分析（PCA）、偏最小二乘法-判别分析（PLS-DA）及t检验考察SS组与健康组间激素水平差异.结果 PLS-DA分析结果表明,6项雄激素可以良好区分SS组与健康组样本.相比较,SS组睾酮（T）（0.17±0.02）ng/mL、二氢睾酮（DHT）（103.72±14.81）ng/mL、脱氢表雄酮（DHEA）（2.7±0.29）ng/mL、雄酮（0.08±0.01）ng/mL及脱氢表雄酮硫酸酯（DHEAS）（795.93±91.68）ng/mL水平,均低于健康组的（0.80±0.08）ng/mL、（150.65±9.92）ng/mL、（4.04±0.28）ng/mL、（0.15±0.02）ng/mL、（1 360.88±76.10）ng/mL,差异均有统计学意义（t=8.536、2.438、3.172、4.158、4.489,均P〈0.05）.PLS-DA分析也可以分别对绝经前及绝经后的两组样本加以区分.结论 通过LC-MS检测血清中6种雄激素,获得了SS组与健康组的差异性雄激素,可为SS诊疗带来新的思路.Objective To evaluate the clinical value of 6 androgens in the serum of Sj？gren＇s syndrome （SS） measured by liquid chromatography tandem mass spectrometry （LC-MS）.Methods 32 SS patients （SS group） and 25 healthy people （healthy group） were included in this study.6 androgens in the serum were analyzed by LC-MS after prepared.PCA,PLS-DA models and t-test were used to class differentiation of androgens between two groups.Results The results of PLS-DA showed that SS group and healthy group could be well classed by 6 androgens.The levels of testerone （T）,dihydrotestosterone （DHT）,dehydroepiandrosterone （DHEA）,androsterone and DHEAS in SS group were significantly lower than those in healthy group （t=8.536,2.438,3.172,4.158,4.489,all P〈0.05）.The samples before or after menopause could be distinguished between the two groups by PLS-DA.Conclusion The significant difference of androgens was discovered between SS patients and healthy people via the measurement of 6 androgens in serum.It may arise a new idea for diagnosis and treatment of SS.

关 键 词：血清 雄激素 液相色谱-质谱 干燥综合征 

分 类 号：R446.1[医药卫生—诊断学] R593.2[医药卫生—临床医学]