14-3-3家族在HBV复制的肝癌细胞中的表达研究  

Expression of 14-3-3 family members in HBV-replicated hepatoma carcinoma cells

作　　者：张祥[1] 魏杰[1] 靳鑫[1] 王石磊[1] 阳媛[1] 师悦嫄 牛司强[2] 汪德强[3] 

机构地区：[1]重庆医科大学检验医学院,重庆400016 [2]重庆医科大学附属第一医院检验科,重庆400016 [3]重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆400016

出　　处：《重庆医科大学学报》2017年第7期781-786,共6页Journal of Chongqing Medical University

基　　金：重庆市自然科学基金资助项目(编号:cstc2015jcyj A10003);重庆市卫计委2016年医学科研面上资助项目(编号:2016MSXM001);重庆市教委科学技术研究资助项目(编号:KJ1702022)

摘　　要：目的:研究14-3-3家族在乙型肝炎病毒(hepatitis B virus,HBV)复制的肝癌细胞中的表达。方法:运用q RT-PCR和Western blot分析14-3-3家族在HBV复制细胞系中的表达水平;q RT-PCR、Western blot检测HBV复制在Hep G2细胞中对14-3-3ζ表达的影响;Western blot检测HBV复制在小鼠肝组织中对14-3-3ζ表达的影响。结果:在Hep G2.2.15中,14-3-3η、γ、ζm RNA和蛋白的表达水平较Hep G2对照组明显增加(m RNA:t=16.5、27.86、17.23,P=0.000、0.000、0.000;蛋白:t=35.7、16.97、22.51,P=0.000、0.000、0.000);在Hep AD38(Tet-)中只有14-3-3ζm RNA和蛋白的表达水平较Hep AD38(Tet+)对照组明显增加(m RNA:t=14.27,P=0.000;蛋白:t=12.11,P=0.000);HBV复制能使Hep G2中14-3-3ζm RNA和蛋白的表达显著升高(m RNA:F=98.8,P=0.000;蛋白:F=118.3,P=0.000);HBV复制能使小鼠肝组织中14-3-3ζ蛋白的表达显著升高(F=109.4,P=0.000)。结论:HBV可以促进肝癌细胞内14-3-3η、γ、ζ的表达,这一发现为控制HBV感染的治疗策略提供新的靶点。Objective ：To investigate the expression of 14-3-3 family members in HBV-infected hepatocellular carcinoma （HCC）. Methods：The expression levels of 14-3-3 family members in HBV-replicated cell lines were analyzed by qRT-PCR and Western blot. Both qRT-PCR and Western blot were used to detect the effect of HBV replication on the expression of 14-3-3ζ in HepG2 cells. Western blot was used to investigate the effect of HBV replication on the expression of 14-3-3ζ in mouse liver. Results：The expres- sion＇levels of 14-3-3η, γ, ζ mRNA and protein in HepG2.2.15 were significantly higher than those in HepG2 control group respec- tively （mRNA： t=16.5,27.86, 17.23, P=0.000,0.000,0.000; protein ： t=35.7,16.97,22.5 l, P=0.O00,0.000,0.000）. The levels of 14 -3- 3ζ mRNA and protein in HepAD38（Tet-） were apparently higher than those in HepAD38（Tet＋）（mRNA：t=14.27,P=0.000;protein： t= 12.11, P=-0.000）. HBV replication could obviously increase the expression of 14-3-3ζ mRNA and protein in HepG2 （mRNA：F=98.8, P=0.000;protein：F=118.3 ,P=O.000）. HBV replication could significantly increase the expression of 14-3-3ζ protein in mouse liver tissue. Conclusion：HBV can promote the expression of 14-3-3η, γ and ζ in hepatocarcinoma cells. This finding provides a new target for the treatment strategy for controlling infection of HBV.

关 键 词：乙型肝炎病毒 14-3-3ζ 14-3-3家族 

分 类 号：R512.6[医药卫生—内科学]