HPLC法测定不同产地龙胆中龙胆苦苷的含量  

Determination of Gentiopicroside in Chinese Gentian Root from Different Habitats by HPLC

作　　者：李海滨[1] 谭芳[1] 游燕[1] 

机构地区：[1]桂林市中医医院

出　　处：《河南中医》2017年第6期1111-1112,共2页Henan Traditional Chinese Medicine

基　　金：广西壮族自治区自然科学基金项目(编号:2012011A412)

摘　　要：目的:建立龙胆苦苷含量测定的方法,测定不同产地龙胆中龙胆苦苷的含量,为控制药材质量提供参考。方法:采用Hypersil ODS2 C18色谱柱;以甲醇-水(25∶75)为流动相;流速为1 m L·min-1;检测波长为270 nm,柱温:30℃,测定不同产地龙胆中龙胆苦苷的含量。结果:龙胆苦苷在0.002 15~0.107 5 g·L^(-1)的浓度范围内呈现良好的线性关系;平均回收率为100.07%,RSD为2.32%。结论:该方法快捷、简便、重复性好,通过测定不同产地龙胆药材中龙胆苦苷的含量,为不同产地龙胆质量的控制提供参考。Objective：To establish a method for the content determination of gentiopicroside, to determine the content of gentiopi- croside in Chinese gentian root from different habitats, and to provide reference for quality control of medicinal materials. Meth- ods ： Using Hypersil ODS2 C18 chromatographic column, regarding methanol - water （25 ： 75 ） as mobile phase, the flow rate was I ml/min; the detection wavelength 270nm, column temperature： 30℃, the content of gentiopicroside in different habitats was de- termined. Results：The concentration of gentiopicroside in the range of 0. 002 15 -0. 1075 g · L-1 showed a good linear relation- ship, the average recovery was 100.07%, and RSD was 2.32%. Conclusion：The method is rapid, simple and reproducible. It can provide refernce for the quality control of Chinese gentian root from different habitats by measuring the content of gentiopicroside in Chinese gentian root.

关 键 词：龙胆 龙胆苦苷 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]