免疫荧光和几种特殊染色在肾活检病理诊断中的应用  

Application of Immunofluorescence and other Special Staining in Pathological Diagnosis of Renal Biopsy

作　　者：罗教秀[1] 储兵[1] 曹晓珊[1] 陈应智[1] 吴师珍[1] LUO Jiaoxiu CHU Bing CAO Xiaoshan et al(Zhongshan Affiliated Hospital, Sun Yat-sen University, Guangdong Zhongshan 528403, Chin)

机构地区：[1]中山大学附属中山医院病理科,广东中山528403

出　　处：《河北医学》2017年第6期898-901,共4页Hebei Medicine

基　　金：广东省科技计划项目;(编号:2013B031804)

摘　　要：目的:分析免疫荧光染色及其他几种特殊染色法在肾活检病理诊断中的应用效果。方法:临床纳入58例我院2014年9月至2016年9月期间收治的慢性肾脏疾病患者作为研究对象,所有患者均进行肾穿刺活检,穿刺取得组织采用石蜡制片,分别采用免疫荧光法、六胺银染色法、甲醇刚果红染色法、黏蛋白染色法(PAS)以及Masson三色染色,对比不同方法染色的结果。结果:不同类型肾病患者中,免疫荧光染色中狼疮性肾病中IgA、IgM、IgG、C3以及Clq诊断阳性率明显高于IgA肾病和膜性肾病患者,P<0.05。在不同染色方法的对比中发现,免疫荧光染色法在肾病检测IgA、IgM、IgG、C3以及Clq诊断阳性率明显低于其他染色方法的诊断率,P<0.05。其他染色方法诊断率均无差异,P>0.05。结论:免疫荧光染色相对于其他染色方式对肾活检病理诊断率稍低,临床上推荐采用其他特殊染色方法对肾组织进行病理诊断。Objective：To analyze the effect of immunofluorescence and other special staining methods in the pathological diagnosis of renal biopsy.Methods： 58 patients with chronic kidney disease admitted in our hospital from September 2014 to September 2016 were included in the object of study.All patients underwent renal biopsy puncture to obtain tissue using paraffin, respectively by immunofluorescence, six staining of methanol method, Congo red staining and mucin （PAS） and three Masson dye staining, staining results in different ways.Results： The positive rates of IgA, IgM, IgG, C3 and Clq in the patients with different types of nephropathy were significantly higher than those of IgA nephropathy and membranous nephropathy in patients by immunofluorescence staining.In the comparison of different staining methods, it was found that the positive rate of IgA, IgM, IgG, C3 and Clq detected by immunofluorescence staining was significantly lower than that of other staining methods, P〈0.05.There was no difference in the diagnostic rate of other staining methods, P〉0.05.Conclusion：Comparing with other methods, the rate of pathological diagnosis of renal biopsy is lower than that of other methods.It is recommended to use other special staining methods to diagnose renal tissue.

关 键 词：免疫荧光 六胺银 甲醇刚果红 黏蛋白染色 Masson三色染色诊断 

分 类 号：R692[医药卫生—泌尿科学]