清热利湿饮对HaCaT细胞衰老的影响及其机制分析  

Role and Mechanism of Qingre Lishi Recipe in Senescence of HaCaT Cells

作　　者：孙淑娜[1] 李翔宇[1] 魏海峰[2] 李广远[3] 辛静昕 纪云[1] 张晓杰[1] 

机构地区：[1]山东中医药大学第一临床医学院,济南250000 [2]山东中医药大学,济南250355 [3]中国农业银行山东省分行,济南250001 [4]泰山医学院,泰安271016

出　　处：《中国中西医结合皮肤性病学杂志》2017年第3期220-223,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的观察清热利湿饮对人角质形成细胞株HaCaT细胞衰老的影响,探讨其分子作用机制。方法使用SA-β-Gal(β-半乳糖苷酶)染色法检测清热利湿饮对细胞衰老的影响;Brd U检测细胞DNA合成,Western blot和realtime PCR检测细胞衰老基因的表达情况。结果与对照组相比,清热利湿饮药物处理可明显促进HaCat细胞衰老;Western blot结果示清热利湿可促进p21蛋白表达上调,抑制p21蛋白表达可缓解清热利湿饮导致的细胞衰老。结论清热利湿饮能通过上调p21蛋白表达促进HaCaT细胞衰老。Objective In order to investigate the effect of Qingre Lishi recipe in the senescence of HaCaT ceils, and to analyze its molecular action mechanism. Methods The effect of Qingre Lishi recipe on cell senescence was detected using SA-β-Gal staining; the DNA replication was examined through BrdU incorporation assay; gene expression was detected by Western blot and reahime PCR. Results Compared with the control group, Qingre Lishi recipe treatment significantly promoted senescence in HaCaT cells. Western blot showed that Qingre Lishi recipe increased expression of p21 protein. Moreover, inhibition of p21 effectively alleviated the cell senescence caused by Qingre Lishi recipe. Conclusion Qingre Lishi recipe could promote HaCaT cell senescence by upregulating expression of p21.

关 键 词：清热利湿饮 细胞衰老 P21 

分 类 号：R758.5[医药卫生—皮肤病学与性病学]