椰子SSR分子标记筛选  

Screening of SSR Markers for Coconut

作　　者：吴翼[1] 李静[1] 杨耀东[1] YOU Yi LI Jing YANG Yao-dong(Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences/Halnan Key Laboratory of Tropical Oil Crops Biology,Wenchang,Halnan 571339)

机构地区：[1]中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌571339

出　　处：《安徽农业科学》2017年第17期119-121,130,共4页Journal of Anhui Agricultural Sciences

基　　金：海南省国际合作专项(KJHZ2014-24);农业部热带作物种质资源保护项目(16RZZY-104)

摘　　要：[目的]筛选可用的SSR分子标记,加快椰子分子辅助育种进程。[方法]采用改良CTAB法提取椰子基因组DNA,以基因组DNA为模板,对36对SSR引物进行筛选,以条带清晰、多态性丰富为引物筛选原则。[结果]共筛选出7对具有多态性的引物,其中2对多态性较丰富,可用于后续SSR分子标记分析。[结论]该研究为开展椰子种质资源遗传多样性系统分析提供了SSR引物。[Objective]To accelerate the molecular assisted breeding process of coconut by screening SSR markers.[Method]The genomic DNA was extracted by modified CTAB method,and 36 SSR primers were screened with genomic DNA as template.[Result]A total of 7 pairs of polymorphic primers were screened,of which more than 2 pairs of polymorphism were found,which could be used for subsequent SSR molecular marker analysis.[Conclusion]The study provides the SSR primers for analysis of coconut germplasm genetic diversity system.

关 键 词：椰子 SSR DNA提取 聚丙烯酰胺凝胶电泳 

分 类 号：S667.4[农业科学—果树学]