毛竹PheDof4-1基因克隆及表达分析  

Molecular cloning and expression analysis of the PheDof4-1 gene in moso bamboo(Phyllostachys edulis)

作　　者：刘俊[1] 黄容[1] 程占超 牟少华[1] 李雪平[1] 高健[1] 

机构地区：[1]国际竹藤中心国家林业局竹藤科学与技术重点实验室,北京100102

出　　处：《安徽农业大学学报》2017年第3期398-403,共6页Journal of Anhui Agricultural University

基　　金：国家林业局"948"项目(2014-4-74);国家自然科学基金(31570673)共同资助

摘　　要：Dof(DNA binding with one finger)蛋白是植物特有的一类转录因子,由多基因共同编码,参与植物转录调控、生长发育及胁迫响应等生物学过程。以毛竹为研究对象,克隆了1个Dof基因,命名为Phe Dof4-1,基因长度为1 473 bp,推测其蛋白质产物的分子量是53.2 kDa,等电点为8.05。qRT–PCR结果显示,Phe Dof4-1在低温和250 mmol·L^(-1) Na Cl处理的幼茎中诱导表达,而在20%PEG8000处理的根中表达则受到强烈抑制,在叶片中不同处理条件下呈现不同的表达趋势。研究结果为Phe Dof4-1在非生物胁迫中的作用提供理论依据,为竹子分子育种提供参考。Dof （DNA binding with one finger） proteins forming an important transcriptional factor family encoding by multiple genes are involved in gene transcriptional regulation, development and stress-responses in plants. In this study, one of Dofgenes, PheDof4-1 （Gene ID No. PH01000209G1040） was isolated from moso bamboo and the sequence was 1 473 nt in length. The predicted molecular weight of PheDof4-1 protein was 53.2 kDa, and the p! value was 8.05. The qRT-PCR analysis displayed that the PheDof4-1 transcription level in young stem was in- duced following exposure to cold stress and salt treatment, however, the expression was restrained in the root under drought treatment. PheDof4-1 showed different expression patterns in the leaf under different stress treatments.

关 键 词：毛竹 Dof转录因子 非生物胁迫 表达模式 

分 类 号：S795.7[农业科学—林木遗传育种]