新乌头碱对K562细胞及耐柔红霉素的K562细胞凋亡的诱导作用及其机制  

Induction Effect of Mesaconitine on Apoptosis of K562 and K562/DNR Cells and Related Mechanism

作　　者：关心[1] 杨同华[1] 张超然[1] 冯帅[1] 

机构地区：[1]云南省第一人民医院血液科,昆明650032

出　　处：《华中科技大学学报（医学版）》2017年第3期281-284,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81160483)

摘　　要：目的观察温阳药新乌头碱对白血病细胞K562和K562耐柔红霉素细胞株(K562/DNR)的抑制作用及可能的分子机制。方法应用MTT法,结合流式细胞术检测不同浓度新乌头碱对K562和K562/DNR增殖和凋亡的影响。通过Real-time PCR检测不同浓度新乌头碱作用72h对K562和K562/DNR细胞C/EBPα、Caspase-3、p53凋亡相关基因表达的影响。结果 K562和K562/DNR细胞的增殖抑制率随着新乌头碱浓度增加及作用时间延长而上升,呈浓度、时间依赖性(均P<0.05)。新乌头碱能引起K562和K562/DNR细胞凋亡,且随着药物浓度增加,凋亡率也逐渐升高,差异均有统计学意义(均P<0.05)。Real-time PCR结果显示,经50μmol/L新乌头碱作用72h,K562和K562/DNR细胞的C/EBPα表达下调,Caspase-3、p53表达上调,与正常对照组相比差异均有统计学意义(均P<0.05)。结论新乌头碱能够引起K562和K562/DNR细胞发生凋亡,并且凋亡机制可能与下调C/EBPa基因及上调Caspase-3、p53基因有关。Objective To observe inhibitory effect of mesaconitine in WenYangherbs on leukemia K562 cells and K562 resistant daunorubicin cell line（K562/DNR）and possible molecular mechanism.Methods Effect of different concentrations of mesaconitine on proliferation and apoptosis of K562 and K562/DNR cells was detected by using MTT and flow cytometry.After treatment for 72 h,effect of different concentrations of mesaconitine on C/EBPα,Caspase-3and p53 in K562and K562/DNR cells was detected by real-time PCR.Results The proliferation of K562 and K562/DNR cells was significantly inhibited by mesaconitine in concentration-and time-dependent manner（P〈0.05）.Mesaconitine significantly induced apoptosis of K562 and K562/DNR cells,and the apoptosis rate was increased with increasing of mesaconitine concentration（P〈0.05）.Real-time PCR results showed that after treatment with 50μmol/L mesaconitine for 72 h,C/EBPαwas significantly decreased,Caspase-3and p53 were significantly increased in K562 and K562/DNR cells as compared with the normal control group（P〈0.05）.Conclusion Mesaconitine can induce apoptosis of K562 cells and K562/DNR cells,and the underlying mechanism is related with downregulation of C/EBPαand upregulation of Caspase-3and p53.

关 键 词：新乌头碱 白血病 细胞凋亡 C/EBPα基因 CASPASE-3基因 P53基因 

分 类 号：R285.5[医药卫生—中药学] R979.14[医药卫生—中医学]