系统性红斑狼疮患者中miR-410表达及其靶基因生物信息学分析  

miR-410 expression and bioinformatics analysis of its predicted target genes in systemic lupus erythematosus

作　　者：张济[1] 欧大明[1] 黄丽芳[1] 欧阳琳[1] 邹礼衡[1] ZHANG Ji OU Da-Ming HUANG Li-Fang OU Yang-Lin ZOU Li-Heng(Department of Rheumatism, the First Affiliated Hospital of University of South China ,Hengyang 421001, China)

机构地区：[1]南华大学附属第一医院风湿免疫科,衡阳421001

出　　处：《中国免疫学杂志》2017年第6期884-888,共5页Chinese Journal of Immunology

基　　金：国家自然科学青年基金(No.81301774);湖南省自然科学基金(No.2016JJ2108)

摘　　要：目的:检测miR-410在系统性红斑狼疮患者(SLE)中的表达水平,运用生物信息学方法研究miR-410及其靶基因在SLE发生发展过程中的作用。方法:定量检测miR-410在SLE患者外周血单个核细胞中的表达水平,并通过miR-410序列分析,靶基因预测和Genecards数据库分析,进一步对其靶基因进行GO富集和KEGG Pathway分析。结果:miR-410在SLE患者中表达显著降低,其核苷酸序列在多物种间呈高度保守性。受miR-410调控且与SLE疾病相关的潜在靶基因包括FASLG、CSF2、IFNAR2、MAPK1、PLCG2、IL4等。GO分析发现miR-410的靶基因参与细胞生长增殖、程序性死亡、细胞分化、免疫系统发育等多个生物过程。KEGG Pathway分析发现miR-410的靶基因显著富集在肿瘤途径信号通路、细胞因子-细胞因子受体相互作用信号通路、胶质瘤信号通路、黑色素瘤信号通路、TGF-β和JAK-STAT信号通路等。结论:miR-410可能通过直接靶向作用其调控靶分子,影响SLE患者体内多条信号通路的网络调控,从而参与SLE疾病的发生和发展。Objective： To detect expression level of miR-410 in patients with systemic lupus erythematosus （SLE）, and to expose the role of miR-410 and its target genes by bioinformatics methods. Methods： Expression level of miR-410 were detected by quantitative RT-PCR in peripheral blood mononuclear cells of SLE patients, and miR-410 sequence, its target genes and Genecards database were analyzed, and analysis of GO enrichment and KEGG Pathway was further performed. Results：miR-410 expression was significantly reduced in SLE patients, and its nucleotide sequence was highly conserved among species. These genes that were predicted to be regulated by miR-410 and associated with LE pathogenesis, included FASLG, CSF2, IFNAR2, MAPK1, PLCG2, IIA and other genes. Analysis of GO enrichment revealed that miR-dl0＇s target genes were involved in cell growth, proliferation, programmed cell death, cell differentiation, immune system development and other biological activities. Analysis of KEGG Pathway showed that the target genes of miR-410 were significantly enriched in a series of signaling pathways including pathways in cancer,cytokine-cytokine receptor interaction, glioma, melanoma, TGF-β and JAK-STAT signaling pathway. Conclusion： miR-410 maybe directly regulate its target molecules, mediate various signal pathway networks, thus participate in the occurrence and development of SLE.

关 键 词：miR-410 SLE 靶基因 GO富集 KEGG信号通路 

分 类 号：R593.2[医药卫生—内科学]