胰岛素和川芎嗪联合治疗对严重烫伤内毒素血症大鼠血浆sTREM-1、sICAM-1、MDA和SOD水平的影响  

Effects of Insulin Combined with Tetramethylpyrazine Therapy on Plasma sTREM-1,sICAM-1,MDA and SOD Levels of Rats with Endotoximia after Severe Scalding Injury

作　　者：杨洋[1,2] 陈和平[1] 王占科[1,3] 祝仲珍 张晓云 王庆莅[3] 熊圆圆 

机构地区：[1]南昌大学医学院,南昌330006 [2]解放军94医院药学科,南昌330002 [3]解放军94医院检验科,南昌330002 [4]解放军94医院感染管理科,南昌330002 [5]解放军184医院检验科,江西鹰潭335000

出　　处：《解放军医药杂志》2017年第6期10-14,18,共6页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：江西省卫计委课题(20173025)

摘　　要：目的探讨胰岛素和川芎嗪单独和联合治疗对严重烫伤内毒素血症大鼠血浆可溶性髓样细胞触发蛋白-1(sTREM-1)、可溶性细胞间黏附分子-1(sICAM-1)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平影响,观察其抗炎和抗氧化效果。方法建立重度烫伤内毒素血症SD大鼠模型80只,按随机数字法随机分为胰岛素组(腹腔注射胰岛素)、川芎嗪组(腹腔注射川芎嗪)、联合组(腹腔注射胰岛素和川芎嗪)及对照组(腹腔注射生理盐水),各20只。测定各组伤前,伤后1、3、5和7 d血浆sTREM-1、ICAM-1、MDA和SOD水平变化。结果 4组伤后各时间点血sTREM-1和sICAM-1以及MDA水平均高于伤前(P<0.01),SOD水平低于伤前(P<0.01)。胰岛素组、川芎嗪组和联合组伤后各时间点血浆sTREM-1、sICAM-1以及MDA水平均低于对照组(P<0.01),SOD水平均高于对照组(P<0.01)。联合组伤后各时间点血浆sTREM-1、sICAM-1和MDA水平均低于胰岛素组和川芎嗪组(P<0.01),SOD水平均明显高于胰岛素组和川芎嗪组(P<0.01)。结论严重烫伤内毒素血症大鼠存在过度炎性反应和氧自由基损伤,胰岛素和川芎嗪具有抗炎和抗氧化作用,且联合治疗比单独治疗效果好;胰岛素和川芎嗪联合治疗可作为防治严重烫伤后过度炎性反应和氧化应激损伤的有效药物。Objective To discuss effects of Insulin combined with Tetramethylpyrazine （TMP） therapy and every single one therapy on plasma soluble triggering receptor expressed on myeloid cells-1 （ sTREM-1 ） , soluble intercellu- lar adhesion molecule-1 （ slCAM-1 ） , superoxide dismutase （SOD） malondialdehyde and （MDA） of rats with endotoxim- ia after severe scalding injury, and to observe their anti-inflammatory and anti-oxidization effects. Methods A total of 80 SD rat models with endotoximia after severe scalding injury were established, and were randomly divided into Insulin group （n = 20, intraperitoneal injection of Insulin） , TMP group （n = 20, intraperitoneal injection of TMP） , combined group （ n = 20, intraperitoneal injection of Insulin and TMP） and control group （ n = 20, intraperitoneal injection of phys- iological saline）. Changes of plasma sTREM-1, ICAM-1, MDA and SOD levels were detected before and at 1~＇, 3rd , 5~h and 7~ d after injury in all groups. Results In the 4 groups, STREM-1, sICAM-1 and MDA levels at all time points af- ter injury were significantly higher （P 〈0.01 ） , while SOD levels were significantly lower than those before injury （P 〈 0.01 ）. In Insulin group, TMP group and combined group, plasma sTREM-1, sICAM-1 and MDA levels were significant- ly lower （ P 〈 0.01 ） , while SOD levels were significantly higher at all time point after injury than those in control group （P 〈 0. 01 ）. In combined group, plasma sTREM-1, sICAM-1 and MDA levels were significantly lower （P 〈 0.01 ） , while SOD levels were significantly higher than those in Insulin and TMP groups （ P 〈 0.01 ）. Conclusion Rats with endotoximia after sever scalding injury have exceeding inflammatory reaction and oxygen free radical damage. Insulin and TMP have anti-inflammatory and anti-oxidization effects, and the effect of combined therapy is better than those by every single one, and therefore Insulin combined with TMP can be used as an effective medication fo

关 键 词：胰岛素 川芎嗪 烧伤 内毒素血症 大鼠 Sprague-Dawley 炎症 氧化性应激 

分 类 号：R644[医药卫生—外科学] R332[医药卫生—临床医学]