人脐带间质干细胞来源的外泌体对顺铂抗肿瘤疗效的影响  

Effects of exosomes from human umbilical cord mesenchymal stem cells on cisplatin chemotherapy

作　　者：王娟娟[1] 贾浩源[1] 张斌[1] 俞静[1] 纪成[1] 毛飞[1] 许文荣[1] 钱晖[1] 

机构地区：[1]江苏大学医学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2017年第3期190-193,199,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81272481);江苏省高校自然科学研究重大项目(15KJA320001);江苏高校优秀科技创新团队(SJK2013-10)

摘　　要：目的:探讨人脐带间质干细胞来源的外泌体(exosome from human umbilical cord mesenchymal stem cells,hucMSC-ex)对顺铂抗肿瘤效果的影响,评价hucMSC-ex的安全性。方法:蛋白质印迹法检测外泌体的表面特异性分子CD9、CD63以鉴定hucMSC-ex。通过细胞抑制率测定、克隆形成实验、细胞迁移实验,检测hucMSC-ex存在条件下顺铂对HGC-27细胞和MGC-803细胞抑制率、克隆形成及迁移效率的影响。结果:HucMSC-ex表达外泌体特异性分子标志物CD9、CD63;hucMSC-ex能减弱顺铂对HGC-27细胞和MGC-803细胞的生长抑制作用,但不影响顺铂作用后的HGC-27细胞和MGC-803细胞克隆形成及迁移效率。结论:hucMSC-ex对顺铂抗肿瘤疗效无明显影响,具有一定的安全性。Objective：To explore the effects of exosome from human umbilical cord mesenchymal stem cells（hucMSC-ex） on cisplatin chemotherapy and to assess the safety of hucMSC-ex.Methods：The specific molecular marker of exosome were detected by Western blotting.By detecting the inhibitor rate, clone formation and migration efficiency of cisplatin to HGC-27 and MGC-803 with treatment of hucMSC-ex to explore the effects of hucMSC-ex.Results：HucMSC-ex expressed the specific marker CD9 and CD63.HucMSC-ex could affect the inhibitor rate of cisplatin to HGC-27 and MGC-803 cells slightly.However, hucMSC-ex did not influence the the clone formation and migration efficiency of cisplatin to HGC-27 and MGC-803 cells.Conclusion：Exosome from human umbilical cord mesenchymal stem cells exerted minor influence on cisplatin chemotherapy and were relatively safe.

关 键 词：hucMSC-ex 顺铂 化疗 影响 

分 类 号：R-052[医药卫生]