稻瘟病菌不同生长期蛋白精氨酸甲基转移酶基因的表达分析  

Expression Analysis of Protein Arginine Methyltransferase Genes in Magnaporthe oryzae

作　　者：吴立叶 王国梁[1] 刘文德[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100193

出　　处：《生物技术通报》2017年第6期104-111,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31422045)

摘　　要：精氨酸甲基化是一种广泛存在于真核生物中的蛋白质翻译后修饰,其主要过程为蛋白质精氨酸甲基转移酶(Protein arginine methyltransferases,PRMTs)催化S-腺苷甲硫胺酸(S-adenosylmethionine,SAM)提供的甲基基团转移到蛋白质精氨酸侧链胍基基团上。由稻瘟病菌(Magnaporthe oryzae)侵染水稻引起的稻瘟病,每年均会为水稻生产造成严重损失。近年来稻瘟病菌和水稻已成为研究植物病原真菌与寄主植物互作的理想模式生物。生物信息学分析表明,稻瘟病菌中含有4个PRMT基因(Mo PRMT1-4),经蛋白序列比对分析发现,它们均含有保守的甲基转移酶结构域;构建系统发育树,表明稻瘟病菌PRMTs在丝状真菌中高度保守;通过提取稻瘟病菌在不同生长和侵染时期的m RNA,利用实时荧光定量PCR技术,分析稻瘟病菌PRMTs基因的表达谱,发现Mo PRMT1在侵染后24 h表达量达到最高峰,而其他阶段表达水平基本一致;Mo PRMT2与其他3个基因相比,各阶段表达量均较低,且各时期表达水平也没有明显变化;Mo PRMT3在芽管和附着胞发育阶段表达量较高,Mo PRMT3和Mo PRMT4在成熟附着胞时期表达量均达到最高,Mo PRMT4在侵染后42 h也出现表达峰值。这些数据表明PRMTs基因对于稻瘟病菌的侵染致病可能起重要调控作用。Arginine methylation is a kind of post-translational modifications existing widely in eukaryotes,of which the main processis to transfer methyl groups provided by S-adenosylmethionine（SAM）to the guanidino group of protein arginine side chain while catalyzedby protein arginine methyltransferases（PRMTs）. Rice blast fungus（Magnaporthe oryzae）infects rice under the suitable conditions,causing severe rice blast and resulting in serious loss of rice yield per year. In recent years,rice blast fungus and rice have already becomethe ideal model for the study of interaction between plant pathogenic fungi and their host plants. Bioinformatics analysis showed that the riceblast fungus contained four PRMT genes（Mo PRMT1-4）,and PRMTs protein sequence alignment revealed that they contained conservativemethyltransferase domains. The PRMTs were demonstrated to be very conservative in filamentous fungi through phylogenetic tree analysis.Extracting the m RNAs of Mo PRMT1-4 at various growth and infection stages,we used real time fluorescence quantitative PCR to analyze theexpression profiles of the genes,and discovered that the expression of Mo PRMT1 reached peak at 24 h post infection,while almost the same atother stages. Compared with other three genes,the expression of Mo PRMT2 was low and showed no obvious variation at each stage. Mo PRMT3 expressed in high level at germ tube and appressorium developmental stages,while both Mo PRMT3 and Mo PRMT4 reached peak expressionsat mature appressorium stage,and Mo PRMT4 also presented peak at 42 h post infection. These data implied that PRMTs genes may play animportant regulation role in the pathogenicity of M. oryzae.

关 键 词：稻瘟病菌 PRMTs 表达分析 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]